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Metabolome and exposome profiling of the biospecimens from COVID-19 patients in India (Nasopharyngeal swab assay)

ABSTRACT:

INTRODUCTION: COVID-19 has become a global impediment by bringing everything to a halt starting from January 2020. India underwent the lockdown starting from 22nd March 2020 with the sudden spike in the number of COVID-19 patients in major cities and states. This study focused on how metabolites play a crucial role in SARSCoV-2 prognosis.

MATERIALS AND METHODS: Metabolome profiling of 106 plasma samples and 24 swab samples from symptomatic patients in the Indian population of the Mumbai region was done. COVID-19 positive samples were further segregated under the non-severe COVID-19 and severe COVID-19 patient cohort for both plasma and swab.

RESULTS: After analyzing the raw files, total 7,949 and 12,871 metabolites in plasma and swab were found. 11 and 35 significantly altered metabolites were found in COVID-19 positive compared to COVID-19 negative plasma and swab samples, respectively. Also, 9 and 23 significantly altered metabolites were found in severe COVID-19 positive to non-severe COVID-19 positive plasma and swab samples, respectively. The majorly affected pathways in COVID-19 patients were found to be the amino acid metabolism pathway, sphingosine metabolism pathway, and bile salt metabolism pathway.

CONCLUSION: This study facilitates identification of potential metabolite-based biomarker candidates for rapid diagnosis and prognosis for clinical applications.

Nasopharyngeal swab assay is reported in the current study MTBLS2349.

Blood plasma assay is reported in MTBLS2291.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Nirjhar Banerjee

PROVIDER: MTBLS2349 | MetaboLights | 2022-01-13

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS2349	Other
	FILES	Other
	a_MTBLS2349_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt

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Metabolome and exposome profiling of the biospecimens from COVID-19 patients in India (Blood plasma assay)

Project description:INTRODUCTION: COVID-19 has become a global impediment by bringing everything to a halt starting from January 2020. India underwent the lockdown starting from 22nd March 2020 with the sudden spike in the number of COVID-19 patients in major cities and states. This study focused on how metabolites play a crucial role in SARSCoV-2 prognosis.MATERIALS AND METHODS: Metabolome profiling of 106 plasma samples and 24 swab samples from symptomatic patients in the Indian population of the Mumbai region was done. COVID-19 positive samples were further segregated under the non-severe COVID-19 and severe COVID-19 patient cohort for both plasma and swab.RESULTS: After analyzing the raw files, total 7,949 and 12,871 metabolites in plasma and swab were found. 11 and 35 significantly altered metabolites were found in COVID-19 positive compared to COVID-19 negative plasma and swab samples, respectively. Also, 9 and 23 significantly altered metabolites were found in severe COVID-19 positive to non-severe COVID-19 positive plasma and swab samples, respectively. The majorly affected pathways in COVID-19 patients were found to be the amino acid metabolism pathway, sphingosine metabolism pathway, and bile salt metabolism pathway.CONCLUSION: This study facilitates identification of potential metabolite-based biomarker candidates for rapid diagnosis and prognosis for clinical applications. Blood plasma assay is reported in the current study MTBLS2291.Nasopharyngeal swab assay is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS2349' rel='noopener noreferrer' target='_blank'>MTBLS2349</a>.

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