Dataset Information

Lipid mediators of inflammation in BALF 3-11 days after infection with influenza

ABSTRACT: Bioactive lipid mediators play a crucial role in the induction and resolution of inflammation. To elucidate their involvement during influenza infection, liquid chromatography/mass spectrometry lipidomic profiling of 141 lipid species was performed on a mouse influenza model using two viruses of significantly different pathogenicity. Infection by the low-pathogenicity strain X31/H3N2 induced a proinflammatory response followed by a distinct anti-inflammatory response; infection by the high-pathogenicity strain PR8/H1N1 resulted in overlapping pro- and anti-inflammatory states. Integration of the large-scale lipid measurements with targeted gene expression data demonstrated that 5-lipoxygenase metabolites correlated with the pathogenic phase of the infection, whereas 12/15-lipoxygenase metabolites were associated with the resolution phase. Hydroxylated linoleic acid, specifically the ratio of 13- to 9-hydroxyoctadecadienoic acid, was identified as a potential biomarker for immune status during an active infection. Importantly, some of the findings from the animal model were recapitulated in studies of human nasopharyngeal lavages obtained during the 2009-2011 influenza seasons.

INSTRUMENT(S): API 4000 QTRAP (AB Sciex)

SUBMITTER: Peter Askovich

PROVIDER: MTBLS33 | MetaboLights | 2013-05-30

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS33	Other
	FILES	Other
	a_MTBLS33_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS33_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Lipidomic profiling of influenza infection identifies mediators that induce and resolve inflammation.

Tam Vincent C VC Quehenberger Oswald O Oshansky Christine M CM Suen Rosa R Armando Aaron M AM Treuting Piper M PM Thomas Paul G PG Dennis Edward A EA Aderem Alan A

Cell 20130701 1

Bioactive lipid mediators play a crucial role in the induction and resolution of inflammation. To elucidate their involvement during influenza infection, liquid chromatography/mass spectrometry lipidomic profiling of 141 lipid species was performed on a mouse influenza model using two viruses of significantly different pathogenicity. Infection by the low-pathogenicity strain X31/H3N2 induced a proinflammatory response followed by a distinct anti-inflammatory response; infection by the high-patho ...[more]

PMID: 23827684

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