Untargeted Metabolomics Reveals a Mild Impact of Remote Ischemic Conditioning on the Plasma Metabolome and α-Hydroxybutyrate as a Possible Cardioprotective Factor and Biomarker of Tissue Ischemia

ABSTRACT: Remote ischemic conditioning (RIC) is a maneuver by which short non-lethal ischemic events are applied on distant organs or limbs to reduce ischemia and reperfusion injuries caused by e.g. heart attacks. As RIC can be induced using a simple blood pressure cuff e.g. prior to revascularization, it constitutes an easily applicable, safe and low-cost intervention to limit these injuries. Although intensively investigated, the specific mechanism of this protective phenomenon remains incompletely understood. Likewise, the identity of circulating factors, presumed to be involved in the initiation of protection, is unknown. In this study, we aimed to study perturbations in the plasma metabolome following RIC and gain insight into metabolic changes by the intervention as well as to identify potential novel cardio-protective metabolites. Blood plasma samples from 10 healthy males were collected prior to and after RIC and analyzed using untargeted LC-qTOF-MS. The analysis revealed a moderate impact on the plasma metabolome following RIC. However, one metabolite, α-hydroxybutyrate (AHB), stood out as highly significantly upregulated after RIC. AHB might be a novel and more sensitive plasma-biomarker of transient tissue ischemia than lactate. Importantly, it was also found that a cell permeable AHB precursor protects cardiomyocytes from ischemia-reperfusion damage.

INSTRUMENT(S): maXis (Bruker)

SUBMITTER: Mogens Johannsen 

PROVIDER: MTBLS414 | MetaboLights | 2016-12-20

REPOSITORIES: MetaboLights