Dataset Information

Lactobacillus plantarum MR1 alleviates high-carbohydrate diet-induced oxidative stress and liver lipid deposition via upregulating uridine synthesis

ABSTRACT: The overconsumption of carbohydrates induces oxidative stress and lipid accumulation in the liver, which can be alleviated by modulation of intestinal microbiota; however, the underlying mechanism remains unclear. Here, we demonstrated that a strain affiliated with Lactobacillus plantarum (designed as MR1) efficiently attenuated lipid deposition, oxidative stress, as well as inflammatory response, which are caused by high-carbohydrate diet (HC) in fish with poor utilization ability of carbohydrates. Serum untargeted metabolome analysis indicated that pyrimidine metabolism was the significantly changed pathway among the groups. In addition, the content of serum uridine was significantly decreased in the HC group compared with the control group, while it increased by supplementation with L. plantarum MR1. Further analysis showed that addition of L. plantarum MR1 reshaped the composition of gut microbiota and increased the content of intestinal acetate. In vitro experiment showed that sodium acetate could induce the synthesis of uridine in hepatocytes. Furthermore, we proved that uridine could directly ameliorate oxidative stress and decrease liver lipid accumulation in the hepatocytes. In conclusion, this study indicated that probiotic L. plantarum MR1 ameliorated high-carbohydrate diet-induced hepatic lipid accumulation and oxidative stress by increasing the circulating uridine, suggesting that intestinal microbiota can regulate the metabolism of nucleotides to maintain host physiological homeostasis.

INSTRUMENT(S): Liquid Chromatography MS - positive, Liquid Chromatography MS - negative

SUBMITTER: Rong Xu

PROVIDER: MTBLS4262 | MetaboLights | 2024-01-16

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS4262	Other
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	a_MTBLS4262_LC-MS_negative__metabolite_profiling.txt	Txt
	a_MTBLS4262_LC-MS_positive__metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt

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<i>Lactobacillus plantarum</i> Ameliorates High-Carbohydrate Diet-Induced Hepatic Lipid Accumulation and Oxidative Stress by Upregulating Uridine Synthesis.

Xu Rong R Wang Tong T Ding Fei-Fei FF Zhou Nan-Nan NN Qiao Fang F Chen Li-Qiao LQ Du Zhen-Yu ZY Zhang Mei-Ling ML

Antioxidants (Basel, Switzerland) 20220624 7

The overconsumption of carbohydrates induces oxidative stress and lipid accumulation in the liver, which can be alleviated by modulation of intestinal microbiota; however, the underlying mechanism remains unclear. Here, we demonstrated that a strain affiliated with <i>Lactobacillus plantarum</i> (designed as MR1) efficiently attenuated lipid deposition, oxidative stress, as well as inflammatory response, which are caused by high-carbohydrate diet (HC) in fish with poor utilization ability of car ...[more]

PMID: 35883730

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