Metabolomics

Dataset Information

0

Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureus


ABSTRACT:

Pathogenic bacteria's metabolic adaptation for survival and proliferation within hosts is a crucial aspect of bacterial pathogenesis. Here, we demonstrate that citrate, the first intermediate of the tricarboxylic acid (TCA) cycle, plays a key role as a regulator of gene expression in Staphylococcus aureus. We show that citrate activates the transcriptional regulator CcpE and thus modulates the expression of numerous genes involved in key cellular pathways such as central carbon metabolism, iron uptake and the synthesis and export of virulence factors. Citrate can also suppress the transcriptional regulatory activity of ferric uptake regulator. Moreover, we determined that accumulated intracellular citrate, partly through the activation of CcpE, decreases the pathogenic potential of S. aureus in animal infection models. Therefore, citrate plays a pivotal role in coordinating carbon metabolism, iron homeostasis and bacterial pathogenicity at the transcriptional level in S. aureus, going beyond its established role as a TCA cycle intermediate.

INSTRUMENT(S): Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

SUBMITTER: Chen Feifei 

PROVIDER: MTBLS4276 | MetaboLights | 2024-04-26

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS4276 Other
FILES Other
a_MTBLS4276_LC-MS_negative_hilic_metabolite_profiling.txt Txt
a_MTBLS4276_LC-MS_positive_hilic_metabolite_profiling.txt Txt
i_Investigation.txt Txt
Items per page:
1 - 5 of 8

Similar Datasets

2017-06-30 | GSE100668 | GEO
2021-11-06 | GSE188170 | GEO
2011-11-29 | GSE25977 | GEO
2010-07-01 | E-GEOD-22052 | biostudies-arrayexpress
2021-04-14 | PXD023797 | JPOST Repository
2011-11-29 | E-GEOD-25977 | biostudies-arrayexpress
2010-07-01 | GSE22052 | GEO
2016-11-02 | GSE89267 | GEO
2020-03-19 | GSE144533 | GEO
2014-09-09 | E-GEOD-54900 | biostudies-arrayexpress