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Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit

ABSTRACT: Rice (Oryza sativa L.) is a candidate crop for production of plant-based vaccines by genetic engineering technologies. MucoRice-CTB has been developed as a rice-seed-based vaccine against cholera by transgenic expression of modified cholera toxin B-subunit (CTB) under the RNA interference (RNAi)-mediated suppression of endogenous seed storage proteins. Here, we performed non-targeted metabolomic profiling of MucoRice-CTB to understand the overall effects of the genetic engineering on rice seed metabolism using gas chromatography/time-of-flight mass spectrometry.

INSTRUMENT(S): GCT Premier (Waters)

SUBMITTER: Takumi Ogawa

PROVIDER: MTBLS437 | MetaboLights | 2017-08-30

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS437	Other
	FILES	Other
	a_MTBLS437_seed_metabolome_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS437_seed_metabolome_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit.

Ogawa Takumi T Kashima Koji K Yuki Yoshikazu Y Mejima Mio M Kurokawa Shiho S Kuroda Masaharu M Okazawa Atsushi A Kiyono Hiroshi H Ohta Daisaku D

Scientific reports 20170712 1

Plant-based human vaccines have been actively developed in recent years, and rice (Oryza sativa L.) is one of the best candidate crops for their production and delivery. By expressing a modified cholera toxin B (CTB) subunit, we previously developed MucoRice-CTB, a rice-based vaccine against cholera, which is caused by infection of the intestine with the bacteria Vibrio cholerae. MucoRice-CTB lines have been extensively characterized by whole-genome sequencing and proteome analyses to evaluate t ...[more]

PMID: 28701756

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