Dataset Information

An Unbiased Lipid Phenotyping Approach To Study the Genetic Determinants of Lipids and Their Association with Coronary Heart Disease Risk Factors

ABSTRACT: Direct infusion high-resolution mass spectrometry (DIHRMS) is a novel, high-throughput approach to rapidly and accurately profile hundreds of lipids in human serum without prior chromatography, facilitating in-depth lipid phenotyping for large epidemiological studies to reveal the detailed associations of individual lipids with coronary heart disease (CHD) risk factors. Intact lipid profiling by DIHRMS was performed on 5662 serum samples from healthy participants in the Pakistan Risk of Myocardial Infarction Study (PROMIS). We developed a novel semi-targeted peak-picking algorithm to detect mass-to-charge ratios in positive and negative ionization modes. We analyzed lipid partial correlations, assessed the association of lipid principal components with established CHD risk factors and genetic variants, and examined differences between lipids for a common genetic polymorphism. The DIHRMS method provided information on 360 lipids (including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids), with a median coefficient of variation of 11.6% (range: 5.4-51.9). The lipids were highly correlated and exhibited a range of associations with clinical chemistry biomarkers and lifestyle factors. This platform can provide many novel insights into the effects of physiology and lifestyle on lipid metabolism, genetic determinants of lipids, and the relationship between individual lipids and CHD risk factors.

INSTRUMENT(S): Direct infusion MS - positive

SUBMITTER: Cecilia Castro

PROVIDER: MTBLS4461 | MetaboLights | 2024-02-02

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS4461	Other
	FILES	Other
	a_MTBLS4461_DI-MS_alternating__metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS4461_DI-MS_alternating__metabolite_profiling_v2_maf.tsv	Tabular

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An Unbiased Lipid Phenotyping Approach To Study the Genetic Determinants of Lipids and Their Association with Coronary Heart Disease Risk Factors.

Harshfield Eric L EL Koulman Albert A Ziemek Daniel D Marney Luke L Fauman Eric B EB Paul Dirk S DS Stacey David D Rasheed Asif A Lee Jung-Jin JJ Shah Nabi N Jabeen Sehrish S Imran Atif A Abbas Shahid S Hina Zoubia Z Qamar Nadeem N Mallick Nadeem Hayyat NH Yaqoob Zia Z Saghir Tahir T Rizvi Syed Nadeem Hasan SNH Memon Anis A Rasheed Syed Zahed SZ Memon Fazal-Ur-Rehman FU Qureshi Irshad Hussain IH Ishaq Muhammad M Frossard Philippe P Danesh John J Saleheen Danish D Butterworth Adam S AS Wood Angela M AM Griffin Julian L JL

Journal of proteome research 20190426 6

Direct infusion high-resolution mass spectrometry (DIHRMS) is a novel, high-throughput approach to rapidly and accurately profile hundreds of lipids in human serum without prior chromatography, facilitating in-depth lipid phenotyping for large epidemiological studies to reveal the detailed associations of individual lipids with coronary heart disease (CHD) risk factors. Intact lipid profiling by DIHRMS was performed on 5662 serum samples from healthy participants in the Pakistan Risk of Myocardi ...[more]

PMID: 30887811

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