Dataset Information

Metabolomic homeostasis shifts after callus formation and shoot regeneration in tomato

ABSTRACT: Plants can regenerate from a variety of tissues on culturing in appropriate media. However, the metabolic shifts involved in callus formation and shoot regeneration are largely unknown. The metabolic profiles of callus generated from tomato (Solanum lycopersicum) cotyledons and that of shoot regenerated from callus were compared with the pct1-2 mutant that exhibits enhanced polar auxin transport and the shr mutant that exhibits elevated nitric oxide levels. The transformation from cotyledon to callus involved a major shift in metabolite profiles with denser metabolic networks in the callus. In contrast, the transformation from callus to shoot involved minor changes in the networks. The metabolic networks in pct1-2 and shr mutants were distinct from wild type and were rewired with shifts in endogenous hormones and metabolite interactions. The callus formation was accompanied by a reduction in the levels of metabolites involved in cell wall lignification and cellular immunity. On the contrary, the levels of monoamines were upregulated in the callus and regenerated shoot. The callus formation and shoot regeneration were accompanied by an increase in salicylic acid in wild type and mutants. The transformation to the callus and also to the shoot downregulated LST8 and upregulated TOR transcript levels indicating a putative linkage between metabolic shift and TOR signalling pathway. The network analysis indicates that shift in metabolite profiles during callus formation and shoot regeneration is governed by a complex interaction between metabolites and endogenous hormones.

INSTRUMENT(S): LECO-PEGASUS GCXGC-TOF-MS 173

SUBMITTER: Rameshwar Sharma

PROVIDER: MTBLS447 | MetaboLights | 2018-06-29

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS447	Other
	FILES	Other
	a_MTBLS447_metabolomic_homeostasis_during_callusing_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS447_metabolomic_homeostasis_during_callusing_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Metabolomic homeostasis shifts after callus formation and shoot regeneration in tomato.

Kumari Alka A Ray Kamalika K Sadhna Sadhna S Pandey Arun Kumar AK Sreelakshmi Yellamaraju Y Sharma Rameshwar R

PloS one 20170508 5

Plants can regenerate from a variety of tissues on culturing in appropriate media. However, the metabolic shifts involved in callus formation and shoot regeneration are largely unknown. The metabolic profiles of callus generated from tomato (Solanum lycopersicum) cotyledons and that of shoot regenerated from callus were compared with the pct1-2 mutant that exhibits enhanced polar auxin transport and the shr mutant that exhibits elevated nitric oxide levels. The transformation from cotyledon to c ...[more]

PMID: 28481937

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