Dataset Information

Host: Microbiome co-metabolic processing of dietary polyphenols – An acute, single blinded, cross-over study with different doses of apple polyphenols in healthy subjects

ABSTRACT: Apples are one of the most commonly consumed fruits and their high polyphenol content is considered one of the most important determinants of their health-promoting activities. Here we studied the nutrikinetics of apple polyphenols by UHPLC-HRMS metabolite fingerprinting, comparing the bioavailability when assumed in a natural or a polyphenol-enriched cloudy apple juice. Twelve men and women participated in an acute single blind controlled crossover study in which they consumed 250 mL of cloudy apple juice (CAJ), Crispy Pink apple variety, or 250 mL of the same juice enriched with 750 mg of an apple polyphenol extract (PAJ). Plasma and whole blood were collected at time 0, 1, 2, 3 and 5 h. Urine was collected at time 0 and 0-2, 2-5, 5-8, and 8-24 h after juice consumption. Faecal samples were collected from each individual during the study for 16S rRNA gene profiling. As many as 110 metabolites were significantly elevated following intake of polyphenol enriched cloudy apple juice, with large inter-individual variations. The comparison of the average area under the curve of circulating metabolites in plasma and in urine of volunteers consuming either the CAJ or the PAJ demonstrated a stable metabotype, suggesting that an increase in the concentration of polyphenols in the fruit do not limit their bioavailability. Faecal bacteria were correlated with specific microbial catabolites derived from apple polyphenols. Human metabolism of apple polyphenols is a co-metabolic process between human encoded activities and those of our resident microbiota. Here we have identified specific blood and urine metabolic biomarkers of apple polyphenol intake and identified putative associations with specific genera of gut bacteria, associations which now need confirmation in specifically designed mechanistic studies.

INSTRUMENT(S): LTQ XL (Thermo Scientific)

SUBMITTER: Maria Ulaszewska

PROVIDER: MTBLS473 | MetaboLights | 2018-10-11

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS473	Other
	FILES	Other
	a_MTBLS473_NEG_Plasma_mass_spectrometry.txt	Txt
	a_MTBLS473_NEG_Urine_mass_spectrometry.txt	Txt
	a_MTBLS473_POS_Plasma_mass_spectrometry.txt	Txt

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Publications

Host: Microbiome co-metabolic processing of dietary polyphenols - An acute, single blinded, cross-over study with different doses of apple polyphenols in healthy subjects.

Trošt Kajetan K Ulaszewska Maria M MM Stanstrup Jan J Albanese Davide D De Filippo Carlotta C Tuohy Kieran M KM Natella Fausta F Scaccini Cristina C Mattivi Fulvio F

Food research international (Ottawa, Ont.) 20180608

Apples are one of the most commonly consumed fruits and their high polyphenol content is considered one of the most important determinants of their health-promoting activities. Here we studied the nutrikinetics of apple polyphenols by UHPLC-HRMS metabolite fingerprinting, comparing bioavailability when consumed in a natural or a polyphenol-enriched cloudy apple juice. Twelve men and women participated in an acute single blind controlled crossover study in which they consumed 250 mL of cloudy app ...[more]

PMID: 30131118

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