Dataset Information

Pharmacokinetic and urinary profiling reveals prednisolone/cortisol ratio as a valid biomarker for prednisolone administration

ABSTRACT: In Europe, synthetic corticosteroids are not allowed in animal breeding fro growth-promoting purposes. Nevertheless, a high prevalence of non-compliant urine samples was recently reported for prednisolone, however, without any indication of unauthorized use. Within this context, 20b-dihydroprednisolone and the prednisolone/cortisol ratio have been suggested as potential tools to discriminate between exogenous and endogenous urinary prednisolone.
In this study, the validity of these strategies was verified by investigating the urinary excretion profiles of relevant glucocorticoids in bovines, subjected to either 1) exogenous prednisolone treatment per os (PO), 2) exogenous prednisolone treatment by intra-muscular injection (IM), 3) tetracosactide hexaacetate (ACTH) to induce endogenous prednisolone formation, and 4) animals at slaughter to simulate natural stress conditions. For treatments the exogenous treatments, both growth-promoting and therapeutic prednisolone doses were considered. Bovine urine samples were analysed by liquid chromatography and mass spectrometry.

INSTRUMENT(S): Exactive (Thermo Scientific)

SUBMITTER: Beata Pomian

PROVIDER: MTBLS488 | MetaboLights | 2018-01-23

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MTBLS488	Other
	FILES	Other
	a_MTBLS488_bovine_urine_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS488_bovine_urine_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

Items per page:

1 - 5 of 7

Publications

Pharmacokinetic and urinary profiling reveals the prednisolone/cortisol ratio as a valid biomarker for prednisolone administration.

Van Meulebroek Lieven L De Clercq Nathalie N Vanden Bussche Julie J Devreese Mathias M Fichant Eric E Delahaut Philippe P Croubels Siska S Vanhaecke Lynn L

BMC veterinary research 20170814 1

<h4>Background</h4>In Europe, synthetic corticosteroids are not allowed in animal breeding for growth-promoting purposes. Nevertheless, a high prevalence of non-compliant urine samples was recently reported for prednisolone, however, without any indication of unauthorized use. Within this context, 20β-dihydroprednisolone and the prednisolone/cortisol ratio have been suggested as potential tools to discriminate between exogenous and endogenous urinary prednisolone. In this study, the validity of ...[more]

PMID: 28806969

Similar Datasets

Project description:The enteric bacterium Proteus mirabilis is a common cause of complicated urinary tract infections. In the study, microrarrays were used to analyze P. mirabilis gene expression in vivo from experimentally infected mice. Urine was collected at 1, 3, and 7d postinfection, and RNA was isolated from bacteria in the urine for transcriptional analysis. Across 9 microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. Genes upregulated in vivo encoded MR/P fimbriae, urease, iron uptake systems, amino acid and peptide transporters, pyruvate metabolism, and portions of the TCA cycle. Flagella were downregulated. Ammonia assimilation gene glnA (glutamine synthetase) was repressed in vivo while gdhA (glutamate dehydrogenase) was upregulated in vivo. Contrary to our expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene expression. A gdhA mutant was growth-deficient in minimal medium with citrate as the sole carbon source, and loss of gdhA resulted in a significant fitness defect in the mouse model of urinary tract infection. Unlike Escherichia coli, which represses gdhA and upregulates glnA in vivo and cannot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor carbon-nitrogen balance, and this ability contributes to the pathogenic potential of P. mirabilis in the urinary tract. Voided urine from female CBA/J mice infected with Proteus mirabilis was collected and pooled in RNA stabilizing reagent (RNAprotect). Urine was collected at 1, 3, and 7 d postinfection. RNA was isolated from urine and log-phase LB cultures, converted to cDNA, and labeled with CyDye. Three arrays were completed per time point (9 arrays total). Slides were scanned with a ScanArray Express microarray scanner (Perkin Elmer) at 10 μm resolution and quantified using ScanArray Express software. Resulting data were normalized by total intensity and median spot intensities were identified using MIDAS (v. 2.22) software.