Metabolomics,Multiomics

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Complete pathway elucidation and heterologous reconstitution of Rhodiola salidroside biosynthesis


ABSTRACT: Salidroside is a bioactive tyrosine-derived phenolic natural product found in medicinal plants under the Rhodiola genus. In addition to their roles in traditional medicine as anti-fatigue and anti-anoxia adaptogens, Rhodiola total extract and salidroside have also displayed medicinal properties as anti-cardiovascular diseases and anti-cancer agents. The resulting surge in global demand of Rhodiola plants and salidroside has driven some species close to extinction. Here, we report the full elucidation of Rhodiola salidroside biosynthetic pathway utilizing the first comprehensive transcriptomics and metabolomics datasets for Rhodiola rosea. Unlike the previously proposed pathway involving separate decarboxylation and deamination enzymatic steps from tyrosine to the key intermediate 4-hydroxyphenylacetaldehyde, Rhodiola contains a pyridoxal phosphate (PLP)-dependent 4-hydroxyphenylacetaldehyde synthase (4HPAAS) that directly converts tyrosine to 4-hydroxyphenylacetaldehyde. We further identified genes encoding the subsequent 4-hydroxyphenylacetaldehyde reductase (4HPAR) and tyrosol:UDP-glucose 8-O- glucosyltransferase (T8GT), respectively, to complete salidroside biosynthesis in Rhodiola. We show that heterologous production of salidroside can be achieved in yeast Saccharomyces cerevisiae as well as in plant Nicotiana benthamiana through transgenic expression of Rhodiola salidroside biosynthetic genes. This study provides new tools for engineering sustainable production of salidroside in heterologous hosts.

OTHER RELATED OMICS DATASETS IN: PRJNA398393

INSTRUMENT(S): Liquid Chromatography MS - Alternating (LC-MS (Alternating))

SUBMITTER: Tomas Pluskal 

PROVIDER: MTBLS566 | MetaboLights | 2018-01-25

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS566 Other
FILES Other
a_MTBLS566_metabolite_profiling_mass_spectrometry.txt Txt
i_Investigation.txt Txt
m_MTBLS566_metabolite_profiling_mass_spectrometry_v2_maf.tsv Tabular
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Publications

Complete Pathway Elucidation and Heterologous Reconstitution of Rhodiola Salidroside Biosynthesis.

Torrens-Spence Michael P MP   Pluskal Tomáš T   Li Fu-Shuang FS   Carballo Valentina V   Weng Jing-Ke JK  

Molecular plant 20171220 1


Salidroside is a bioactive tyrosine-derived phenolic natural product found in medicinal plants under the Rhodiola genus. In addition to their anti-fatigue and anti-anoxia roles in traditional medicine, Rhodiola total extract and salidroside have also displayed medicinal properties as anti-cardiovascular diseases and anti-cancer agents. The resulting surge in global demand of Rhodiola plants and salidroside has driven some species close to extinction. Here, we report the full elucidation of the R  ...[more]

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