Dataset Information

Creation and Characterization of a Genomically Hybrid Strain in the Nitrogen-Fixing Symbiotic Bacterium Sinorhizobium meliloti

ABSTRACT: Many bacteria, often associated with eukaryotic hosts and of relevance for biotechnological applications, harbor a multipartite genome composed of more than one replicon. Biotechnologically relevant phenotypes are often encoded by genes residing on the secondary replicons. A synthetic biology approach to developing enhanced strains for biotechnological purposes could therefore involve merging pieces or entire replicons from multiple strains into a single genome. Here we report the creation of a genomic hybrid strain in a model multipartite genome species, the plant-symbiotic bacterium Sinorhizobium meliloti. We term this strain as cis-hybrid, since it is produced by genomic material coming from the same species' pangenome. In particular, we moved the secondary replicon pSymA (accounting for nearly 20% of total genome content) from a donor S. meliloti strain to an acceptor strain. The cis-hybrid strain was screened for a panel of complex phenotypes (carbon/nitrogen utilization phenotypes, intra- and extracellular metabolomes, symbiosis, and various microbiological tests). Additionally, metabolic network reconstruction and constraint-based modeling were employed for in silico prediction of metabolic flux reorganization. Phenotypes of the cis-hybrid strain were in good agreement with those of both parental strains. Interestingly, the symbiotic phenotype showed a marked cultivar-specific improvement with the cis-hybrid strains compared to both parental strains. These results provide a proof-of-principle for the feasibility of genome-wide replicon-based remodelling of bacterial strains for improved biotechnological applications in precision agriculture.

INSTRUMENT(S): Nuclear Magnetic Resonance (NMR)

PROVIDER: MTBLS576 | MetaboLights | 2021-03-17

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	CELL-LYSATE-0512-7-1.zip	Other
	CELL-LYSATE-0512-7-2.zip	Other
	CELL-LYSATE-0512-7-3.zip	Other
	CELL-LYSATE-0512-7-4.zip	Other
	CELL-LYSATE-0512-P110-1.zip	Other

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Publications

Creation and Characterization of a Genomically Hybrid Strain in the Nitrogen-Fixing Symbiotic Bacterium Sinorhizobium meliloti.

Checcucci Alice A diCenzo George C GC Ghini Veronica V Bazzicalupo Marco M Becker Anke A Decorosi Francesca F Döhlemann Johannes J Fagorzi Camilla C Finan Turlough M TM Fondi Marco M Luchinat Claudio C Turano Paola P Vignolini Tiziano T Viti Carlo C Mengoni Alessio A

ACS synthetic biology 20181003 10

Many bacteria, often associated with eukaryotic hosts and of relevance for biotechnological applications, harbor a multipartite genome composed of more than one replicon. Biotechnologically relevant phenotypes are often encoded by genes residing on the secondary replicons. A synthetic biology approach to developing enhanced strains for biotechnological purposes could therefore involve merging pieces or entire replicons from multiple strains into a single genome. Here we report the creation of a ...[more]

PMID: 30223644

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