Dataset Information

Assessing the potentiality of sputtered gold nanolayers in mass spectrometry imaging for metabolomics applications (Liver MALDI-MSI assay)

ABSTRACT:

Mass spectrometry imaging (MSI) is a molecular imaging technique that maps the distribution of molecules in biological tissues with high spatial resolution. The most widely used MSI modality is matrix-assisted laser desorption/ionization (MALDI), but some organic matrices used in classical MALDI may impact the quality of the molecular images due to limited lateral resolution and strong background noise in the low mass range, hindering its use in metabolomics. Here we present a matrix-free LDI technique based on the deposition by sputtering of gold nanolayers on tissue sections. This gold coating method is quick, fully automated and repetitive and allows growing highly controlled nanolayers, necessary for high quality and high resolution MS image acquisition. The performance of the developed method has been tested on the acquisition of MS images of brain. The obtained spectra showed a high number of MS peaks on the low mass region (m/z below 1000 Da) with few background peaks, demonstrating the viability of the sputtered gold nanolayers of promoting the desorption/ionization of a wide range of metabolites. These results, together with the reliable MS spectrum calibration using gold peaks, make the developed method a valuable alternative for MSI applications.

Liver MALDI MS imaging assay is reported in the current study MTBLS589.

Brain MALDI MS imaging assay is reported in MTBLS588.

INSTRUMENT(S): ultrafleXtreme MALDI-TOF MS (Bruker)

SUBMITTER: Pere Rafols Soler

PROVIDER: MTBLS589 | MetaboLights | 2022-01-21

REPOSITORIES: MetaboLights

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Assessing the potential of sputtered gold nanolayers in mass spectrometry imaging for metabolomics applications.

Ràfols Pere P Vilalta Dídac D Torres Sònia S Calavia Raul R Heijs Bram B McDonnell Liam A LA Brezmes Jesús J Del Castillo Esteban E Yanes Oscar O Ramírez Noelia N Correig Xavier X

PloS one 20181212 12

Mass spectrometry imaging (MSI) is a molecular imaging technique that maps the distribution of molecules in biological tissues with high spatial resolution. The most widely used MSI modality is matrix-assisted laser desorption/ionization (MALDI), mainly due to the large variety of analyte classes amenable for MALDI analysis. However, the organic matrices used in classical MALDI may impact the quality of the molecular images due to limited lateral resolution and strong background noise in the low ...[more]

PMID: 30540827

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Project description:It has been recognised for a long time that cell surface glycans plays a vital role in the biological process and their altered form can lead to cancer. However, the molecular details and regulatory mechanism between the glycans and cancer is yet not clear. Over the past decade mass spectrometry-based techniques has become a prominent method for analysing glycans especially N-glycan matrix assisted laser desorption/ionisation mass spectrometry imaging (MALDI MSI). It is a powerful technique that combines mass spectrometry with histology, enabling the visualisation and label free detection of N-linked glycans on a single tissue section. Here, we carried out N-glycan MALDI MSI on six endometrial cancer (EC) formalin fixed paraffin embedded (FFPE) tissue sections including the adjacent normal endometrium, and tissue microarrays (TMA) consisting of 8 EC patients with lymph node metastasis (LNM) and 20 without LNM. By doing that several m/z values were detected that can significantly distinguish normal endometrium from cancerous. Also, detected a m/z value that can discriminate the primary tumour with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS). Overall, we observed higher abundance of oligomannose in tumour regions compared to normal with AUC ranges from 0.85-0.99. Whereas, complex N-glycans were detected in lower abundance with AUC ranges from 0.03-0.28. Comparison of N-glycans between the primary tumours with LNM and without LNM indicates reduced abundance of a complex core-fucosylated N-glycan in patients with metastasis relative to without. In summary, N-glycan MALDI MSI can be used to characterize the cancerous endometrium from the normal, also patients with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS).

			Action	DRS
	MTBLS589	Other
	FILES	Other
	a_MTBLS589_MSImaging___metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt