Metabolomics,Multiomics

Dataset Information

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LC-MS metabolic profiling of mouse plasma and cell culture medium in relation to ABCC6 expression


ABSTRACT: Pseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ABCC6 (MRP6), an ATP-dependent efflux transporter that is mainly present in the liver. Abcc6-/- mice have been instrumental in the demonstration that PXE is a metabolic disease caused by the absence of an unknown factor in the circulation, the presence of which depends on ABCC6 in the liver. Why absence of this factor results in PXE remained a mystery. We now show that medium from HEK293 cells overexpressing either human or rat ABCC6 potently inhibits mineralization in vitro, whereas medium of HEK293 control cells does not. Untargeted metabolomics revealed that cells expressing ABCC6 excrete large amounts of nucleoside triphosphates, even though ABCC6 itself did not transport nucleoside triphosphates. Extracellularly, ectonucleotidases hydrolyzed the excreted nucleoside triphosphates to nucleoside monophosphates and inorganic pyrophosphate (PPi), a strong inhibitor of mineralization that plays a pivotal role in several mineralization disorders similar to PXE. The in vivo relevance of our data was demonstrated in Abcc6-/- mice, which had plasma PPi levels that were less than 40% of those found in wild-type mice. This study provides the first insight into how ABCC6 affects PXE. Our data indicate that the factor that normally prevents PXE is PPi, which is provided to the circulation in the form of nucleoside triphosphates via an as yet unidentified, but ABCC6-dependent mechanism.

OTHER RELATED OMICS DATASETS IN: PXD006204

INSTRUMENT(S): LTQ Orbitrap Discovery (Thermo Scientific)

SUBMITTER: Robert Jansen 

PROVIDER: MTBLS61 | MetaboLights | 2013-11-14

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS61 Other
FILES Other
a_MTBLS61_abcc6_mouse_plasma_metabolite_profiling_mass_spectrometry.txt Txt
a_MTBLS61_plasma_metabolite_profiling_mass_spectrometry.txt Txt
i_Investigation.txt Txt
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Publications

ABCC6 prevents ectopic mineralization seen in pseudoxanthoma elasticum by inducing cellular nucleotide release.

Jansen Robert S RS   Küçükosmanoglu Aslı A   de Haas Marcel M   Sapthu Sunny S   Otero Jon Andoni JA   Hegman Ilse E M IE   Bergen Arthur A B AA   Gorgels Theo G M F TG   Borst P P   van de Wetering Koen K  

Proceedings of the National Academy of Sciences of the United States of America 20131125 50


Pseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes, and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ATP-binding cassette sub-family C member 6 (ABCC6), an ATP-dependent efflux transporter present mainly in the liver. Abcc6(-/-) mice have been instrumental in demonstrating that PXE is a metabolic disease caused by the absence of an unknown factor  ...[more]

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