A large-scale metabolomics study to harness chemical diversity and explore biochemical mechanisms in ryegrass (UPLC-MS C1 reverse phase POS and GC-MS FAME assays)

ABSTRACT: Perennial ryegrass (Lolium perenne) is integral to temperate pastoral agriculture, which contributes most of the milk and meat production worldwide. Chemical profiles and diversity of ryegrass offer several opportunities to harness specific traits and elucidate underlying biological mechanisms for forage improvement. We conducted a large-scale metabolomics study of perennial ryegrass comprising 715 genotypes, representing 118 populations from 21 countries. Liquid/gas chromatography-mass spectrometry based targeted and non-targeted techniques were used to analyse fructan oligosaccharides, lipids, fatty acid methyl esters, polar and semi-polar compounds. Fructan diversity across all genotypes was evaluated, high- and low-sugar groups identified, and fructan accumulation mechanisms explored. Metabolites differentiating the two groups were characterised, modules and pathways they represent deduced, and finally, visualisation and interpretation provided in a biological context. We also demonstrate a workflow for large-scale metabolomics studies from raw data through to statistical and pathway analysis.

UPLC-MS C1 reverse phase POS and GC-MS FAME assays are reported in the current study MTBLS66.
UPLC-MS HILIC POS assay is reported in MTBLS62.
UPLC-MS HILIC NEG assay is reported in MTBLS63.
UPLC-MS C18 reverse phase POS assay is reported in MTBLS64.
UPLC-MS C18 reverse phase NEG assay is reported in MTBLS65.
UPLC-MS C1 reverse phase NEG assay is reported in MTBLS68.
HPLC-MS Hypercarb reverse phase NEG assay is reported in MTBLS529.

INSTRUMENT(S): Exactive (Thermo Scientific), DSQ II Trace Ultra (Thermo Scientific)

SUBMITTER: Arvind Subbaraj 

PROVIDER: MTBLS66 | MetaboLights | 2019-03-01

REPOSITORIES: MetaboLights