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Metabolome analysis reveal key regulatory pathways of feed conversion efficiency of oriental river prawn Macrobrachium nipponense

ABSTRACT:

A total of 445 and 247 differently expressed metabolites (DEMs) were identified in the hepatopancreas and muscle. In addition, 11 KEGG pathways, which mainly include lysine biosynthesis, lysine degradation, aminoacyl-tRNA biosynthesis and purine metabolism, were significantly enriched in hepatopancreas. Also, 14 KEGG pathways, which mainly include pyrimidine metabolism, glutathione metabolism, nitrogen metabolism and mTOR signaling pathway, were significantly enriched in muscle. The metabonomic analysis revealed that the feed conversion efficiency of M. nipponense was affected considerably by amino acid, lipid and nucleotide metabolism. Hence, different RFI groups of M. nipponense have various metabolic capability processes. The differences in response to immunity, digestion and absorption of nutrients may be the critical elements related to the variation of RFI.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Feiyue Ling

PROVIDER: MTBLS6663 | MetaboLights | 2023-02-28

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS6663	Other
	FILES	Other
	a_MTBLS6663_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS6663_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other

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Project description:Purpose:In this study, we observed the effect of exogenous melatonin on the proliferation and pathogenicity of Alternaria alternata, judged the biological function of melatonin on Alternaria alternata, and found the key genes of melatonin regulating Alternaria alternata traits at the transcription level by RNA SEQ, and preliminarily revealed its mechanism of action. Methods:SNA medium with melatonin concentration of 50, 100, 500, 1000 and 1500 μ m was prepared and 30 ml plate was made. Take sterilized circular filter paper with a diameter of 1 cm, add 100 UL 50 CFU / cm3 spore suspension, transfer to SNA medium center, and culture upside down for 7 days Results:In this study, 20 metabolic pathways with the most significant KEGG enrichment were selected from the four control groups by P value analysis (Fig. 9). In 500 μ m vs control group, DEGs were mainly enriched in ribosome, glyoxylic acid and dicarboxylic acid metabolism pathways. In 1500 μ m vs control group, the metabolic pathways such as glyoxylic acid and dicarboxylic acid metabolism, valine, leucine and isoleucine degradation, ketone body synthesis and degradation, terpenoid main chain biosynthesis were the most significant; in 500 μ m vs 1500 μ m, DEGs were mainly enriched in eukaryotic ribosome biosynthesis and RNA polymerase pathway; 500 μ m vs 1500 μ M Compared with the control treatment, DEGs were significantly enriched among the three groups, and the pathways were rich, such as amino acid metabolism, glyoxylic acid and dicarboxylic acid metabolism, valine, leucine and isoleucine degradation, glyceride metabolism, glycolysis / gluconeogenesis, ABC transporter and so on. Conclusions:Nine DEGs related to the development and pathogenesis of streptozospora were obtained by transcriptional sequencing. The results showed that melatonin involved aflatoxin biosynthesis (sld1 and sld2, s-adenosine-l-methionine dependent methyltransferase synthesis), MAPK signal pathway (GLP), linoleic acid metabolism (HP1), heme peroxidase synthesis ）The results showed that the metabolism of selenium (THR), protein processing in endoplasmic reticulum (KHP, 30kda heat shock protein synthesis), glycolysis / glycometagenesis (PPL), and synthesis of non reductive poly ketoacetase (NPS) and heme peroxidase (HP2) were also studied. Among them, THR is related to proliferation regulation [49], sld1, sld2, NPs and ppl are related to pathogenicity , GLP is related to colony growth and pathogenicity, KHP is related to stress , HP1 and HP2 are related to antioxidant activity .

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Metabolome analysis reveal key regulatory pathways of feed conversion efficiency of oriental river prawn Macrobrachium nipponense

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