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Transcriptomics and Metabolomics Reveal Alterations in Xenobiotic Metabolism and Appearance of Steroidal Alkaloid Metabolites in Mouse Livers after Tomato Consumption

ABSTRACT:

SCOPE: Tomato consumption is associated with many health benefits including lowered risk for developing certain cancers. It is hypothesized that tomato phytochemicals are transported to the liver and other tissues where they alter gene expression in ways that lead to favorable health outcomes. However, the effects of tomato consumption on mammalian liver gene expression and chemical profile are not well defined.

METHODS AND RESULTS: We hypothesized that tomato consumption would alter mouse liver transcriptomes and metabolomes compared to a control diet. C57BL/6 mice (n=11-12/group) were fed a macronutrient matched diet containing either 10% red tomato, 10% tangerine tomato, or no tomato powder for 6 weeks after weaning. RNA-Seq followed by gene set enrichment analyses indicated that tomato type and consumption, in general, altered expression of phase I and II xenobiotic metabolism genes. Untargeted metabolomics experiments revealed distinct clustering between control and tomato fed animals. Nineteen molecular formulas (representing 75 chemical features) were identified or tentatively identified as steroidal alkaloids and isomers of their phase I and II metabolites; many of which are reported for the first time in mammals.

CONCLUSION: These data together suggest tomato consumption may impart benefits partly through enhancing detoxification potential.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Jessica Cooperstone

PROVIDER: MTBLS6715 | MetaboLights | 2023-06-13

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS6715	Other
	FILES	Other
	a_MTBLS6715_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt

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Transcriptomics and Metabolomics Reveal Liver Metabolic Changes and Phytochemical Deposition Occurring after Tomato Consumption in Mice

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