Dataset Information

Croquemort elicits activation of the immune deficiency pathway in ticks

ABSTRACT: The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins that elicit the IMD pathway in non-insect arthropods remain elusive. Here, we show that an Ixodes scapularis homolog of croquemort (Crq), a CD36-like protein, promotes activation of the tick IMD pathway. Crq exhibits plasma membrane localization and binds the lipid agonist 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. Crq regulates the IMD and jun N-terminal kinase signaling cascades and limits the acquisition of the Lyme disease spirochete B. burgdorferi. Additionally, nymphs silenced for crq display impaired feeding and delayed molting to adulthood due to a deficiency in ecdysteroid synthesis. Collectively, we establish a distinct mechanism for arthropod immunity outside of insects and crustaceans.

INSTRUMENT(S): Q Exactive

SUBMITTER: Joao Pedra

PROVIDER: MTBLS686 | MetaboLights | 2023-04-27

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS686	Other
	FILES	Other
	a_MTBLS686_Method1_POS_1_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS686_Method2_POS_2_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS686_Method3_NEG_1_metabolite_profiling_mass_spectrometry.txt	Txt

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Croquemort elicits activation of the immune deficiency pathway in ticks.

O'Neal Anya J AJ Singh Nisha N Rolandelli Agustin A Laukaitis Hanna J HJ Wang Xiaowei X Shaw Dana K DK Young Brianna D BD Narasimhan Sukanya S Dutta Shraboni S Snyder Greg A GA Samaddar Sourabh S Marnin Liron L Butler L Rainer LR Mendes M Tays MT Cabrera Paz Francy E FE Valencia Luisa M LM Sundberg Eric J EJ Fikrig Erol E Pal Utpal U Weber David J DJ Pedra Joao H F JHF

Proceedings of the National Academy of Sciences of the United States of America 20230508 20

The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins that elicit the IMD pathway in non-insect arthropods remain elusive. Here, we show that an <i>Ixodes scapularis</i> homolog of croquemort (Crq), a CD36-like protein, promotes activation of the tick IMD pathway. Crq exhibits plasma membrane localization and bind ...[more]

PMID: 37155900

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