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N6-methyladenosine modification is not a general trait of positive-sense RNA viral genomes

ABSTRACT:

Despite the nuclear localization of the m6A machinery, the genomes of multiple exclusively-cytoplasmic RNA viruses, such as chikungunya (CHIKV) and dengue (DENV), are reported to be extensively m6A-modified. However, these findings are mostly based on m6A-seq, an antibody-dependent technique with a high rate of false positives. Here, we addressed the presence of m6A in CHIKV and DENV RNAs. For this, we combined m6A-seq and the antibody-independent SELECT and nanopore direct RNA sequencing techniques with functional, molecular, and mutagenesis studies. Following this comprehensive analysis, we found no evidence of m6A modification in CHIKV or DENV transcripts. Furthermore, depletion of key components of the host m6A machinery did not affect CHIKV or DENV infection. Moreover, CHIKV or DENV infection had no effect on the m6A machinery’s localization. Our results challenge the prevailing notion that m6A modification is a general feature of cytoplasmic RNA viruses and underscore the importance of validating RNA modifications with orthogonal approaches.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS6978 | MetaboLights | 2024-01-25

REPOSITORIES: MetaboLights

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			Action	DRS
	2019LZ023_REME_001_CHK_H4a_250ng.raw	Raw
	2020MR001_MOLU_001_01_CHK_H5.raw	Raw
	2020MR001_MOLU_002_01_CHK_H6.raw	Raw
	2020MR001_MOLU_003_01_NonInf_H1a.raw	Raw
	2020MR001_MOLU_004_01_NonInf_H2.raw	Raw

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N6-methyladenosine modification is not a general trait of viral RNA genomes [Dengue]

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N6-methyladenosine modification is not a general trait of viral RNA genomes [CHIKV]

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