Dataset Information
Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase
Ontology highlight
ABSTRACT: Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS) to identify such interactions. Analysis of 33 enzymes from human carbohydrate metabolism identified 830 protein-metabolite interactions, including known regulators, substrates and products as well as previously unreported interactions. We functionally validated a subset of interactions, including the isoform-specific inhibition of lactate dehydrogenase by long-chain acyl-coenzyme A. Cell treatment with fatty acids caused a loss of pyruvate-lactate interconversion dependent on lactate dehydrogenase isoform expression. These protein-metabolite interactions may contribute to the dynamic, tissue-specific metabolic flexibility that enables growth and survival in an ever-changing nutrient environment.
INSTRUMENT(S): Flow injection analysis MS - positive, Flow injection analysis MS - negative
SUBMITTER: Jordan Berg
PROVIDER: MTBLS7237 | MetaboLights | 2023-08-07
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| MTBLS7237 | Other | |||
| FILES | Other | |||
| a_MTBLS7237_FIA-MS_negative__metabolite_profiling.txt | Txt | |||
| a_MTBLS7237_FIA-MS_positive__metabolite_profiling.txt | Txt | |||
| files-all.json | Other |
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Publications
Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase.
Science (New York, N.Y.) 20230309 6636
Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS) to identify such interactions. Analysis of 33 enzymes from human carbohydrate metabolism identified 830 protein-metabolite interactions, including known regulator ...[more]
