Dataset Information

Synergistic metabolism of salivary MUC5B in oral commensal bacteria during early biofilm formation

ABSTRACT:

Bacterial metabolism in oral biofilms is comprised of complex networks of nutritional chains and biochemical regulations. These processes involve both intraspecies and interspecies networks as well as interactions with components from host saliva, gingival crevicular fluid, and dietary intake. In a previous paper, a large salivary glycoprotein, mucin MUC5B, was suggested to promote a dental health-related phenotype in the oral type strain of Streptococcus gordonii DL1, by regulating bacterial adhesion and protein expression. In this study, nuclear magnetic resonance-based metabolomics was used to examine the effects on the metabolic output of monospecies compared to dual species early biofilms of two clinical strains of oral commensal bacteria, S. gordonii and Actinomyces naeslundii, in the presence of MUC5B. The presence of S. gordonii increased colonization of A. naeslundii on salivary MUC5B, and both commensals were able to utilize MUC5B as a sole nutrient source during early biofilm formation. The metabolomes suggested that the bacteria were able to release mucin carbohydrates from oligosaccharide side chains as well as amino acids from the protein core. Synergistic effects were also seen in the dual species biofilm metabolome compared to the monospecies, indicating that A. naeslundii and S. gordonii cooperated in the degradation of salivary MUC5B. A better understanding of bacterial interactions and salivary-mediated regulation of early dental biofilm activity is meaningful for understanding oral biofilm physiology and may contribute to the development of future prevention strategies for biofilm-induced oral disease.

INSTRUMENT(S): Nuclear Magnetic Resonance (NMR) -

SUBMITTER: Anders Bay Nord

PROVIDER: MTBLS8370 | MetaboLights | 2023-10-30

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS8370	Other
	FILES	Other
	a_MTBLS8370_NMR___metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS8370_NMR___metabolite_profiling_v2_maf.tsv	Tabular

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Publications

Synergistic metabolism of salivary MUC5B in oral commensal bacteria during early biofilm formation.

Robertsson Carolina C Svensäter Gunnel G Davies Julia R JR Bay Nord Anders A Malmodin Daniel D Wickström Claes C

Microbiology spectrum 20231019 6

<h4>Importance</h4>The study of bacterial interactions and salivary-mediated regulation of early dental biofilm activity is of interest for understanding oral microbial adaptation to environmental cues and biofilm maturation. Findings in oral commensals can prove useful from the perspectives of both oral and systemic health of the host, as well as the understanding of general microbial biofilm physiology. The knowledge may provide a basis for the development of prognostic biomarkers, or developm ...[more]

PMID: 37855449

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