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Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry

ABSTRACT: One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one mL of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. This data repository contains data related to the exploration of marine habitats

INSTRUMENT(S): Gas Chromatography MS - Positive (GC-MS (Positive))

SUBMITTER: Manuel Liebeke

PROVIDER: MTBLS844 | MetaboLights | 2019-11-15

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS844	Other
	FILES	Other
	a_MTBLS844_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS844_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Cell culture sampling)

Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. Cell culture sampling is reported in the current study.Method development is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS826" target="_blank">MTBLS826</a>The effect of salt and water on metabolite detection is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS839" target="_blank">MTBLS839</a>Environmental sampling is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS844" target="_blank">MTBLS844</a>Method quantification is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS848" target="_blank">MTBLS848</a>Solid phase extraction is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS849" target="_blank">MTBLS849</a>

2019-11-15 | MTBLS843 | MetaboLights

Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry

Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. Solid phase extraction is reported in the current study MTBLS849. Method development is reported in MTBLS826. The effect of salt and water on metabolite detection is reported in MTBLS839. Cell culture sampling is reported in MTBLS843. Environmental sampling is reported in MTBLS844. Method quantification is reported in MTBLS848. Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'>MTBLS826</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'>MTBLS839</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'>MTBLS843</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'>MTBLS844</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'>MTBLS848</a>

2019-11-15 | MTBLS849 | MetaboLights

Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Method development)

Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. Method development is reported in the current study MTBLS826. The effect of salt and water on metabolite detection is reported in MTBLS839. Cell culture sampling is reported in MTBLS843. Environmental sampling is reported in MTBLS844. Method quantification is reported in MTBLS848. Solid phase extraction is reported in MTBLS849. Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'>MTBLS839</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'>MTBLS843</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'>MTBLS844</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'>MTBLS848</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'>MTBLS849</a>

2019-11-15 | MTBLS826 | MetaboLights

Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Method quantification)

Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. Method quantification is reported in the current study MTBLS848. Method development is reported in MTBLS826. The effect of salt and water on metabolite detection is reported in MTBLS839. Cell culture sampling is reported in MTBLS843. Environmental sampling is reported in MTBLS844. Solid phase extraction is reported in MTBLS849. Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'>MTBLS826</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'>MTBLS839</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'>MTBLS843</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'>MTBLS844</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'>MTBLS849</a>

2019-11-15 | MTBLS848 | MetaboLights

Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (The effect of salt and water on metabolite detection)

Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. The effect of salt and water on metabolite detection is reported in the current study MTBLS839. Method development is reported in MTBLS826. Cell culture sampling is reported MTBLS843. Environmental sampling is reported MTBLS844. Method quantification is reported in MTBLS848. Solid phase extraction is reported in MTBLS849. Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'>MTBLS826</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'>MTBLS843</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'>MTBLS844</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'>MTBLS848</a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'>MTBLS849</a>

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