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Evaluation of extraction solvents for untargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR)

ABSTRACT:

INTRODUCTION: The extraction solvent mixtures were optimized for untargeted metabolomics analysis of microbial communities from two laboratory scale activated sludge reactors performing enhanced biological phosphorus removal (EBPR).

OBJECTIVE: To develop a robust and simple analytical protocol to analyse microbial metabolomics from EBPR bioreactors.

METHODS: Extra- and intra-cellular metabolites were extracted using five methods and analysed by ultraperformance liquid chromatography mass spectrometry (UPLC-MS).

RESULTS: The optimal extraction method was biomass specific and methanol:water (1:1 v/v) and methanol:chloroform:water (2:2:1 v/v) were chosen, respectively, for each of the two different bioreactors.

CONCLUSION: Our approach provides direct surveys of the metabolic state of PAO-enriched EBPR communities, showing that extraction methods should be carefully tailored to the microbial community under study

INSTRUMENT(S): Liquid Chromatography MS - Negative (LC-MS (Negative)), Liquid Chromatography MS - Positive (LC-MS (Positive))

SUBMITTER: Nay Min Min Thaw Saw

PROVIDER: MTBLS884 | MetaboLights | 2019-08-16

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS884	Other
	FILES	Other
	a_MTBLS884_NEG_lipid_profiling_mass_spectrometry.txt	Txt
	a_MTBLS884_NEG_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS884_POS_lipid_profiling_mass_spectrometry.txt	Txt

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Evaluation of extraction solvents for untargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR)

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