Ontology highlight
ABSTRACT: Filamentous cell growth is a vital property of fungal pathogens. The mechanisms of filamentation in the emerging multidrug-resistant fungal pathogen Candida auris are poorly understood. Here, we show that exposure of C. auris to glycerol triggers a rod-like filamentation-competent (RL-FC) phenotype, which forms elongated filamentous cells after a prolonged culture period. Whole-genome sequencing analysis reveals that all RL-FC isolates harbor a mutation in the C2H2 zinc finger transcription factor-encoding gene GFC1 (Gfc1 variants). Deletion of GFC1 leads to an RL-FC phenotype similar to that observed in Gfc1 variants. We further demonstrate that GFC1 mutation causes enhanced fatty acid β-oxidation metabolism and thereby promotes RL-FC/filamentous growth. This regulation is achieved through a Multiple Carbon source Utilizer (Mcu1)-dependent mechanism. Interestingly, both the evolved RL-FC isolates and the gfc1Δ mutant exhibit an enhanced ability to colonize the skin. Our results reveal that glycerol-mediated GFC1 mutations are beneficial during C. auris skin colonization and infection.
INSTRUMENT(S): Gas Chromatography MS - positive
SUBMITTER: Yuchen Deng
PROVIDER: MTBLS9053 | MetaboLights | 2024-07-04
REPOSITORIES: MetaboLights
Action | DRS | |||
---|---|---|---|---|
MTBLS9053 | Other | |||
FILES | Other | |||
a_MTBLS9053_GC-MS_positive__metabolite_profiling.txt | Txt | |||
i_Investigation.txt | Txt | |||
m_MTBLS9053_GC-MS_positive__metabolite_profiling_v2_maf.tsv | Tabular |
Items per page: 5 1 - 5 of 6 |