Metabolomics

Dataset Information

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A single gene mutation underpins metabolic adaptation and acquisition of filamentous competence in the emerging fungal pathogen Candida auris


ABSTRACT:

Filamentous cell growth is a vital property of fungal pathogens. The mechanisms of filamentation in the emerging multidrug-resistant fungal pathogen Candida auris are poorly understood. Here, we show that exposure of C. auris to glycerol triggers a rod-like filamentation-competent (RL-FC) phenotype, which forms elongated filamentous cells after a prolonged culture period. Whole-genome sequencing analysis reveals that all RL-FC isolates harbor a mutation in the C2H2 zinc finger transcription factor-encoding gene GFC1 (Gfc1 variants). Deletion of GFC1 leads to an RL-FC phenotype similar to that observed in Gfc1 variants. We further demonstrate that GFC1 mutation causes enhanced fatty acid β-oxidation metabolism and thereby promotes RL-FC/filamentous growth. This regulation is achieved through a Multiple Carbon source Utilizer (Mcu1)-dependent mechanism. Interestingly, both the evolved RL-FC isolates and the gfc1Δ mutant exhibit an enhanced ability to colonize the skin. Our results reveal that glycerol-mediated GFC1 mutations are beneficial during C. auris skin colonization and infection.

INSTRUMENT(S): Gas Chromatography MS - positive

SUBMITTER: Yuchen Deng 

PROVIDER: MTBLS9053 | MetaboLights | 2024-07-04

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS9053 Other
FILES Other
a_MTBLS9053_GC-MS_positive__metabolite_profiling.txt Txt
i_Investigation.txt Txt
m_MTBLS9053_GC-MS_positive__metabolite_profiling_v2_maf.tsv Tabular
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