Metabolomics

Dataset Information

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Multi-Omics and Integrated Network Analyses Reveal New Insights into the Systems Relationships between Metabolites, Structural Genes, and Transcriptional Regulators in Developing Grape Berries (Vitis vinifera L.) Exposed to Water Deficit (Carotenoids; UPLC-DAD assay)


ABSTRACT:

Grapes are one of the major fruit crops and they are cultivated in many dry environments. This study comprehensively characterizes the metabolic response of grape berries exposed to water deficit at different developmental stages. Increases of proline, branched-chain amino acids, phenylpropanoids, anthocyanins, and free volatile organic compounds have been previously observed in grape berries exposed to water deficit. Integrating RNA-sequencing analysis of the transcriptome with large-scale analysis of central and specialized metabolites, we reveal that these increases occur via a coordinated regulation of key structural pathway genes. Water deficit-induced up-regulation of flavonoid genes is also coordinated with the down-regulation of many stilbene synthases and a consistent decrease in stilbenoid concentration. Water deficit activated both ABA-dependent and ABA-independent signal transduction pathways by modulating the expression of several transcription factors. Gene-gene and gene-metabolite network analyses showed that water deficit-responsive transcription factors such as bZIPs, AP2/ERFs, MYBs, and NACs are implicated in the regulation of stress-responsive metabolites. Enrichment of known and novel cis-regulatory elements in the promoters of several ripening-specific/water deficit-induced modules further affirms the involvement of a transcription factor cross-talk in the berry response to water deficit. Together, our integrated approaches show that water deficit-regulated gene modules are strongly linked to key fruit-quality metabolites and multiple signal transduction pathways may be critical to achieve a balance between the regulation of the stress-response and the berry ripening program. This study constitutes an invaluable resource for future discoveries and comparative studies, in grapes and other fruits, centered on reproductive tissue metabolism under abiotic stress.


UPLC-DAD assay of Carotenoids is reported in the current study MTBLS984

SPME-GC-MS assay of Volatile organic compounds is reported in MTBLS982

UPLC-MS/MS assay of Phenolics is reported in MTBLS898

INSTRUMENT(S): Diode array detection MS - positive - reverse phase

SUBMITTER: Stefania Savoi 

PROVIDER: MTBLS984 | MetaboLights | 2021-08-16

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS984 Other
FILES Other
a_MTBLS984_LC-DAD_positive_reverse-phase_metabolite_profiling.txt Txt
i_Investigation.txt Txt
m_MTBLS984_LC-DAD_positive_reverse-phase_metabolite_profiling_v2_maf.tsv Tabular
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Publications

Multi-Omics and Integrated Network Analyses Reveal New Insights into the Systems Relationships between Metabolites, Structural Genes, and Transcriptional Regulators in Developing Grape Berries (<i>Vitis vinifera</i> L.) Exposed to Water Deficit.

Savoi Stefania S   Wong Darren C J DCJ   Degu Asfaw A   Herrera Jose C JC   Bucchetti Barbara B   Peterlunger Enrico E   Fait Aaron A   Mattivi Fulvio F   Castellarin Simone D SD  

Frontiers in plant science 20170710


Grapes are one of the major fruit crops and they are cultivated in many dry environments. This study comprehensively characterizes the metabolic response of grape berries exposed to water deficit at different developmental stages. Increases of proline, branched-chain amino acids, phenylpropanoids, anthocyanins, and free volatile organic compounds have been previously observed in grape berries exposed to water deficit. Integrating RNA-sequencing analysis of the transcriptome with large-scale anal  ...[more]

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