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Impact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model

ABSTRACT: We examined the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Heart, Liver, Muscle, Adipose Tissue, Blood

SUBMITTER: Charles Evans

PROVIDER: ST000121 | MetabolomicsWorkbench | Fri Nov 21 00:00:00 GMT 2014

REPOSITORIES: MetabolomicsWorkbench

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