Metabolomics

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13C targeted metabolomics


ABSTRACT: Jurkat parental cells (JP6) or cells stably expressing the pCDNA Noxa vector (N5) cells were cultured to confluence and counted. 20E6 cells in triplicate were resuspended in glucose free or glutamine free medium for 2 hours. Cells were then pelleted and resuspended in 13C-1,2 Glucose (10mM) or 13C-U-glutamine (4mM) for 24 hours. Cells were pelleted and spent medium was collected. Cell pellets were washed 1X with ice cold PBS and cell pellets were resuspended in 400ul -20 methanol. Cells and media were snap frozen in liquid nitrogen and stored at -80.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): T-cells

SUBMITTER: Maureen Kachman  

PROVIDER: ST000147 | MetabolomicsWorkbench | Fri Mar 13 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

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