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Comparative metabolomics study of WT and iButOH tolerant E. coli

ABSTRACT: This study aims to elucidate metabolic mechanisms of tolerance to isobutanol in E. coli. Two strains are utilized: WT parental strain EcHW24, and isobutanol tolerant strain 38-20-4 created via multiplex genome engineering. The metabolic response to growth with isobutanol (0.7% w/v) and without (0% w/v) on NG50 minimal media will be compared for each strain. 3 replicates for each strain/iButOH concentration have been submitted, excpet for WT/0.7%; we have observed high growth phenotype variation for this combination, and have corresponding submitted 5x replicates. Strain 38-20-4 contains mutations in the TCA cycle (gltA SNP) and amino acid metabolism (loss-of-function indels in glnE, gltD, and tnaA), thus our hypothesis is that tolerance arises via rewiring of TCA cycle and amino acid metabolism, possibly resulting in increased intracellular ratio of glutamine:glutamate

ORGANISM(S): Escherichia Coli E. Coli

TISSUE(S): Bacterial Cells

SUBMITTER: Maureen Kachman

PROVIDER: ST000173 | MetabolomicsWorkbench | Thu Jun 04 00:00:00 BST 2015

REPOSITORIES: MetabolomicsWorkbench

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