Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Early in life exposure studies on human and mouse samples

ABSTRACT: Experiment1: 2 day old baby mice were exposed to hyperoxia (75% O2) continuously for 7 days. Control baby mice were housed in room air (normoxia). Plasma and bronchoalveolar lavage fluid (BALF) were harvested after 7 days of exposure (on Day of life 9). Experiment2: 2 day old baby mice were exposed to room air or hyperoxia for 14 days and subsequently treated with RV1 or sham.Plasma was collected 5 days after treatment. Human tracheal aspirates were collected from prematurely born infants undergoing mechanical ventilation for respiratory distress syndrome in the first week of life.Tracheal aspirate supernatants are submitted for the assay. We would like to measure adenosine, AMP, ADP and ATP levels.

ORGANISM(S): Human Homo Sapiens Mouse Mus Musculus;homo Sapiens Mus Musculus

TISSUE(S): Lung

SUBMITTER: Maureen Kachman

PROVIDER: ST000304 | MetabolomicsWorkbench | Thu Mar 26 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	mwtab	Other

Items per page:

1 - 1 of 1

Similar Datasets

Baby Mice Hyperoxia treatment (G64-N.H-7d14d)

Project description:In this experiment 2 day old baby mice were exposed to hyperoxia (75% O2) continuously for 14 days. Control baby mice were housed in room air (normoxia). Plasma and bronchoalveolar lavage fluid (BALF) were harvested after 14 days of exposure (on Day of life 16)

2014-10-28 | ST000169 | MetabolomicsWorkbench

Impact of Sphingosine Kinase-1 knock out in neonatal mice lungs

Project description:Sphingosine Kinase-1 knock out protects against hyperoxic lung injury One day old Wild type (WT) control and Sphingosine Kinase-1 knock out (SphK-1 KO)pups exposed to room air (RA) or hyperoxia (HO). Microarray based profiling of lung tissue after 7 days of hyperoxia of 75%

2016-10-30 | GSE87350 | GEO

Expression profiles of mRNAs in the retinas of OIR wildtype, OIR IL-19 knockout and room air control mice.

Project description:Seven-day-old C57BL/6 mice and IL-19 knockout (KO) mice were subjected to 75% oxygen for 5 days to induce OIR. To explore the mechanism that caused by IL-19 on retinal neovascularization, Gene expressions among retinas of room air control mice, OIR wildtype mice, and OIR IL-19 knockout mice were measured by RNA-sequencing (RNA-seq).

2022-01-24 | GSE194176 | GEO

Gene expression after 1 and 5 days of cigarette smoke exposure in mice with chronically inflamed or healthy lungs

Project description:These studies tested the hypotheses that smoke induces changes in mRNA profiles that are dependent on sex and the health status of the lung, and that the effects of smoke are different after 1 day compared to 5 days of smoke exposure. The ways in which the lungs modulate their response to cigarette smoke after repeated exposures are important for understanding the toxicology of smoke, for developing biomarkers of chronic smoke exposure, and for understanding the therapeutic potential in regulatory signaling pathways that are beneficial or detrimental to lung health. Sex-matched 5-7-week old wildtype (WT) and Scnn1b-overexpressing (BENaC) littermates were exposed to cigarette smoke or sham (room air) exposure. Exposure occurred in a plexiglass chamber attached to a smoke delivery device using an exposure chamber and smoking machine (inExpose Exposure System, SCIREQ, Chandler, AZ). Mice were exposed to mainstream + sidestream smoke from 6 reference cigarettes with filters removed per day (3R4F research cigarettes, University of Kentucky). Each cigarette was puffed for 2 sec every 25 sec, using the standard Federal Trade Commission smoking machine protocol. The sham-exposed control mice were exposed to room air in the exposure chamber for a time equivalent to that needed for active smoke exposure. Mice were exposed to cigarette or sham smoke for 1 day or 5 consecutive days. Samples were harvested 4 hours after the completion of the final smoke exposure. The right lung was used for gene expression analysis.

2019-03-18 | GSE109776 | GEO

RNA-sequencing of the oxygen induced retinopathy (OIR) model in C57BL/6 mice

Project description:Pathological retinal angiogenesis is one of the major causes of vision loss worldwide. The breakdown of blood vessels and aberrant vessels growth usually lead to hemorrhage, macular edema, fibrotic scar, and retinal detachment. OIR model serves as a proxy for human pathological retinal neovascularization such as proliferative diabetic retinopathy, retinal vein occlusion and retinopathy of prematurity. C57BL/6 mice were exposed to 75% O2 in a hyperoxic chamber from postnatal day P7 to P12 and then returned to room air. The greatest neovascular response occurred at postnatal 17 days (P17) when the mice are put back to room air condition for 5 days. In order to reveal potential genes that involve in pathological neovascularization, we performed transcriptomic analyses of retina of P17.

2024-08-20 | GSE241239 | GEO

Expression profiling of microRNAs in oxygen-induced retinal neovascularization in mice

Project description:The purpose of this study is to identify disease-related miRNAs in retinas of a mouse model of oxygen-induced retinopathy (OIR). OIR pups were exposed to 75% oxygen at postnatal day (P)7 for 5 days, and were returned to room air at P12. The miRNAs expression profiles in the retinas from OIR mice at P17 and room air controls were determined through microarray analysis. Expressions of significantly upregulated and downregulated miRNAs in the OIR retinas and controls were confirmed through quantitative real-time RT-PCR (qPCR). Compared to the room air controls, 3 miRNAs were significantly up-regulated, and 8 miRNAs were down-regulated in OIR retinas. Our findings indicated that several miRNAs were differentially expressed in the oxygen-induced retinal neovascularization, which might provide novel therapeutic targets in regulating retinal neovascular diseases.

2018-06-19 | GSE115949 | GEO

E-cigarette Aerosol Reduces Left Ventricular Function in Adolescent Mice

Project description:Purpose: to determine the cardiac complications of vaping in adult (3 month old) and juvenile (3 week old) FVB mice. Methods: mice were exposed to filtered air or vape with or without nicotine for 4 hrs/day, 5 days/week for a total of 3 months time. RNA-seq. was conducted on RNA isolated from the left ventricle of mice from each group.

2021-12-25 | GSE183614 | GEO

Gene expression signature induced by 8 weeks of cigarette smoke exposure in the lung of BALB/c mice

Project description:To investigate the impact of cigarette smoke on lung gene expression, female BALB/c mice were obtained from Charles River at 6-8 weeks of age. Using a whole body exposure system (SIU48, PROMECH LAB AB, Vintrie, Sweden), mice (5 per group) were exposed to room air or the mainstream cigarette smoke of twelve 3R4F reference cigarettes (University of Kentucky, Lexington, USA) with filters removed 5 days per week, twice daily for 50 minutes/exposure for 8 weeks, as previously described. Control animals were exposed to room air only. Lungs were collected and stored in RNALater at -80°C prior to RNA extraction.

2014-04-01 | GSE55127 | GEO

Circadian_IHC_ApoE_KO_mice_acute_study

Project description:ApoE knockout mice were exposed to intermittent hypoxia and hypercapnia (treatment) or room air (controls). Perturbations in the gut microbiome were profiled longitudinally using LC-MS/MS to study the impact of treatment on the gut metabolome over the course of the day. Samples were collected after 6 days of exposure to conditions. Samples were collected every 4 hours for 24hrs (6 timepoints) to examine circadian rhythm dynamics.

2020-01-27 | MSV000084847 | MassIVE

Sex-specific differences in the pulmonary transcriptome in neonatal hyperoxic lung injury

Project description:Changes in the pulmonary transcriptome in male and female mice on PND7 and PND21 exposed to room air or hyperoxia

2017-10-27 | GSE97804 | GEO