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Streptococcus mutans organic acids/amino acid profiles, effect of oxidative stress; pilot

ABSTRACT: S. mutans UA159 and ΔspxA1 will be grown in BHI to OD600 = 0.4. Each culture will be split in to 4 samples. For the first sample, 3E10 cells will immediately be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. The remaining three samples will be treated with H2O2 to 0.5 mM, and the harvesting process repeated after 15, 30 and 60 minutes.

ORGANISM(S): Streptococcus Mutans Streptococcus

TISSUE(S): Bacterial Cells

DISEASE(S): Oxidative Stress

SUBMITTER: Emily Hardin

PROVIDER: ST000466 | MetabolomicsWorkbench | Thu Sep 08 00:00:00 BST 2016

REPOSITORIES: MetabolomicsWorkbench

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