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Inhibition of autophagy/proteasome degradation or inhibition of protein synthesis in models of muscle insulin resistance affect amino acids metabolites in serum

ABSTRACT: To determine which protein degradation pathways downstream of IR and IGF1R contribute to changes in amino acid and mitochondrial metabolite pools, we will treat control, M-IR-/-, MIGIRKO, and HFD obese mice with inhibitors of autophagy or proteasome. We will treat 5 animals each of control, M-IR-/-, MIGIRKO, and HFD mice with vehicle, colchicine to inhibit autophagy, or MG132 to inhibit proteasome activity, then measure amino acid metabolites in serum.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Muscle

DISEASE(S): Diabetes

SUBMITTER: Brian O'Neill

PROVIDER: ST000514 | MetabolomicsWorkbench | Fri Dec 02 00:00:00 GMT 2016

REPOSITORIES: MetabolomicsWorkbench

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Determine how inhibition of autophagy/proteasome degradation or inhibition of protein synthesis in models of muscle insulin resistance affect amino acid metabolites

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