Metabolomic analysis of C2C12 myoblasts induced by the transcriptional factor FOXO1
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ABSTRACT: The transcriptional factor FOXO1 is considered to play roles in the regulation of energy metabolism in various tissues. To determine the metabolic changes occurring due to the activation of FOXO1, we analyzed the metabolic profile of C2C12 myoblasts expressing FOXO1-estrogen receptor fusion protein using CE-TOFMS. In the FOXO1-activated cells, the metabolite levels during glycolysis were higher. In addition, the gene expression of pyruvate dehydrogenase kinase, an enzyme that inhibits glucose utilization, increased. In the FOXO1-activated cells, the metabolite levels of numerous amino acids decreased, with increased gene expression of branched chain amino acid metabolism enzymes. Our results suggest that FOXO1 suppresses glucose utilization and promotes the use of proteins/amino acids as energy sources in muscle cells, potentially during starvation.
ORGANISM(S): Mouse Mus Musculus
TISSUE(S): Muscle
SUBMITTER: Yasutomi Kamei
PROVIDER: ST001178 | MetabolomicsWorkbench | Mon Apr 22 00:00:00 BST 2019
REPOSITORIES: MetabolomicsWorkbench
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