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Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice

ABSTRACT: Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression. Since the liver is the main gluconeogenic organ, we performed here liver metabolomics in young (6 months) versus old (20-24 months) WT and SIRT6-transgenic mice. We used liver tissues of 6h morning-fasted mice, either in naïve state or 15 minutes after intraperitoneal injection of 1mg/kg [U-13C]Lactate.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Liver

SUBMITTER: Chaim Cohen

PROVIDER: ST001507 | MetabolomicsWorkbench | Tue Oct 06 00:00:00 BST 2020

REPOSITORIES: MetabolomicsWorkbench

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