Project description:We compared the myocardial transcriptional profiles in male and female rats after CLP (Cecal ligation and puncture) induction and landiolol administration. This analysis showed major differences between males and females in the biological processes activated during sepsis. In particular, a very significant decrease in processes related to cell organization, contractile function, ionic transport and PI3K-Akt signaling were observed only in males. Regarding the direct regulation of gene expression of the adrenergic pathway, there was no major differences between male and female. Moreover, our results highlighted the large number of signaling pathways dysregulated during sepsis in males that were reversed by landiolol. In particular, the level of expression of the genes encoding the contractile proteins, TUBA8 and MYH7B, the phosphatase PPP2CA, GRK5 and AKAP6 were close to their basal levels in males. In contrast, in females, only few pathways were affected by the action of landiolol. In addition, adrenergic signaling was decreased while genes potentially involved in calcium overload were overexpressed.
Project description:To demonstrate that the P. multistriata gene MRP3 is responsible for sex determination, we overexpressed it in a mating type minus strain. The transgenic strain generated displayed sex reversal and behaved like a strain of the opposite mating type. In this study, we compared the gene expression profile of the wild type versus the transformed strain.
Project description:Analysis of CGTH-W-1 follicular thyroid carcinoma cells transcriptome following 48 hrs siRNA-mediated depletion of PROX1. PROX1 is a homeobox transcription factor. PROX1 depletion decreases migratory ability, motility and invasivness and induces profound cytoskeleton changes of CGTH-W-1 cells. Results provide insight into the role of PROX1 in the thyroid cancer. Three biological replicates for a given condition
Project description:We profiled the transcriptomic changes that occur in the Drosophila third antennal segment after silencing the second order olfactory neurons in the antennal lobe with Kir2.1 (EH1). A non-conducting variant of Kir2.1 (Kir2.1-nc or EH2) served as our control.