Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Free fatty acid analysis of NIH-3T3 cells treated with apoptotic inducers

ABSTRACT: Changes of free fatty acid profiles in mouse NIH-3T3 fibroblasts treated with mechanistically diverse apoptotic inducers for 48h.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Fibroblast Cells

SUBMITTER: Andreas Koeberle

PROVIDER: ST001751 | MetabolomicsWorkbench | Fri Apr 02 00:00:00 BST 2021

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	mwtab	Other

Items per page:

1 - 1 of 1

Similar Datasets

Timecourse of NIH-3T3 cells treated with apoptotic inducers

Project description:Time-dependent changes of phosphatidylinositol profiles in mouse NIH-3T3 fibroblasts treated with mechanistically diverse apoptotic inducers.

2021-04-01 | ST001740 | MetabolomicsWorkbench

RNA-seq of NIH 3T3 and HepG2 cells after arsenite treatment

Project description:Expression profiling of HepG2 human liver carcinoma cells and NIH 3T3 mouse fibroblasts after arsite treatment for 24h. RNA-seq data comprise 4 groups: NIH 3T3 mouse fibroblasts control and arsite treatment, and HepG2 human liver carcinoma cells control and arsenite treatment. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

2021-10-19 | GSE117815 | GEO

Immediate Early Response 2 (Ier2) overexpression effect in NIH 3T3 cells

Project description:Analysis of Immediate Early Response 2 (Ier2)-inducible NIH 3T3 cells after Ier2 induction with RheoSwitch ligand RSL-1. Results provide insight into the function of Ier2 in NIH 3T3 mouse embryonal fibroblasts. Immediate early genes, including Ier2, are rapidly induced in quiescent cells by proliferation and migration-inducing stimuli. Microarray gene expression profiling was performed to identify differentially expressed genes following overexpression of Ier2 in NIH 3T3-Ier2 inducible cells after 24 hour induction of Ier2.

2021-10-07 | GSE181200 | GEO

Experimental identification of miR-378-3p targets by overexpression and silencing in murine NIH-3T3 fibroblasts

Project description:MicroRNAs (miRNAs) are short noncoding RNA molecules regulating the expression of mRNAs. Target identification of miRNAs is computationally difficult due to the relatively low homology between miRNAs and their targets. We provide data here utilizing an experimental approach to identify targets of mmu-miR-378-3p, where mmu-miR-378-3p was overexpressed and silenced in NIH-3T3 murine fibroblasts and compared to control RNA transfected cells (RISC-free siRNA). Expression of mRNAs was profiled and differentially expressed genes following either treatment as compared to control transfected cells were identified. In this way we identified 491 significantly differentially expressed genes with more than 1.4 fold change in either comparison. One of the putative targets Akt-1 was subsequently confirmed by luciferase reporter assay. All conditions were assayed in triplicates. A commercially available mimic or inhibitor of mmu-miR-378-3p or control RNA (RISC-free siRNA) were transfected into NIH-3T3 fibroblasts using a chemical transfection system (DharmaFECT 1). 48h post transfection total RNA was isolated and mRNA-expression profiled.

2012-08-07 | E-GEOD-34873 | biostudies-arrayexpress

RNA-Seq of murine NIH/3T3 cells upon Aldoa knockdown

Project description:Aldoa knockdown (using a pool of 4 siRNAs) vs. non-targeting control knockdown (using a pool of 4 siRNAs) in murine NIH/3T3 cells (fibroblasts). Cells were harvested 48 hours after transfection.

2022-07-13 | GSE207899 | GEO

RNA-Seq of NIH-3T3 cells treated with CMA modulators

Project description:Transcriptomics of NIH-3T3 cells treated with CMA activators to evaluate gene expression changes

2022-06-06 | GSE194292 | GEO

The NIH Human Pluripotent Stem Cell Database (Agilent, mRNA)

Project description:To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov.

2012-10-31 | GSE32923 | GEO

RNA-Seq of NIH-3T3 cells treated with atypical RARa modulators

Project description:Transcriptomics of NIH-3T3 cells treated with typical or atypical RARa modulators to evaluate genetic changes induced by chemical modification of RARa signalling

2022-06-06 | GSE194291 | GEO

Apoptotic cells induce proliferation of peritoneal macrophages

Project description:The interaction of macrophages with apoptotic cells is required for efficient resolution of inflammation. While apoptotic cell removal prevents inflammation due to secondary necrosis, it also alters the macrophage phenotype to hinder further inflammatory reactions. The interaction between apoptotic cells and macrophages is often studied by chemical or biological induction of apoptosis, which may introduce artifacts by affecting the macrophages as well and/or triggering unrelated signaling pathways. Here, we set up a pure cell death system in which NIH 3T3 cells expressing dimerizable Caspase-8 were co-cultured with peritoneal macrophages in a transwell system. Phenotype changes in macrophages induced by apoptotic cells were evaluated by RNA sequencing, which revealed an unexpectedly dominant impact on macrophage proliferation. This was confirmed in functional assays with primary peritoneal macrophages and IC-21 macrophages. Moreover, inhibition of apoptosis during Zymosan-induced peritonitis in mice decreased mRNA levels of cell cycle mediators in peritoneal macrophages. Proliferation of macrophages in response to apoptotic cells may be important to increase macrophage numbers to allow efficient clearance and resolution of inflammation.

2021-03-18 | GSE164709 | GEO