Capybara gut microbiome
Ontology highlight
ABSTRACT: The largest living rodent dwelling Pantanal wetlands and Amazon basin, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, combining multi-meta-omics approaches, carbohydrate enzymology and X-ray crystallography, we elucidated the microbial community composition and structure, enzymatic systems and metabolic pathways involved in the conversion of recalcitrant dietary fibers into short-chain fatty acids, a main energy source for the host. The high efficiency of this microbiota in the deconstruction of plant polysaccharides is underpinned on the combination of unique enzymatic mechanisms from Fibrobacteres to degrade cellulose with a broad arsenal of Carbohydrate-Active enZymes (CAZymes) organized in polysaccharide utilization loci (PULs) from Bacteroidetes, to tackle with complex hemicelluloses typically found in gramineous and aquatic plants. Exploring the genomic dark matter of this community, two novel CAZy families were unveiled including a glycoside hydrolase family of β-galactosidases and a carbohydrate-binding module family involved in xylan binding that establishes an unprecedented three-dimensional fold among associated modules to CAZymes. Together, these results demonstrate at community and molecular levels how the capybara gut microbiota orchestrates the deconstruction and utilization of dietary fibers, representing an untapped reservoir of new and intricate enzymatic mechanisms to overcome the lignocellulose recalcitrance, a central challenge toward a bio-based and sustainable economy.
ORGANISM(S): Capybara Hydrochoerus Hydrochaeris
TISSUE(S): Intestine
SUBMITTER: Gabriela Persinoti
PROVIDER: ST001945 | MetabolomicsWorkbench | Sat Sep 18 00:00:00 BST 2021
REPOSITORIES: MetabolomicsWorkbench
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