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Glutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine

ABSTRACT: 107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Macrophages

SUBMITTER: Wei Zhou

PROVIDER: ST002061 | MetabolomicsWorkbench | Wed Jan 19 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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