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Addressing batch effects in large-scale metabolomics with augmented experimental design and meta-analysis (Part 2)

ABSTRACT: Non-polar and polar sequential extracts were collected on three groups of C. elegans: natural isolates, central metabolism mutants, and UDP-glucuronosyltrasnferase mutants. Untargeted LC-MS (HILIC/RP - positive and negative mode) and NMR (CDCl3/H2O) studies were conducted using a Vanquish UHPLC coupled to an Orbitrap Elite MS and NEO 800 MHz Bruker NMR spectrometer, respectively. An augmented design, rank transformation of the raw data, strict feature filtering and QA/QC followed by a meta-analysis was performed on all datasets to demonstrate the benefits of this analysis approach and identify stable, significant features to prioritize for downstream compound identification efforts.

ORGANISM(S): Caenorhabditis Elegans C. Elegans

TISSUE(S): Worms

SUBMITTER: Brianna Garcia

PROVIDER: ST002096 | MetabolomicsWorkbench | Fri Mar 04 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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Addressing batch effects in large-scale metabolomics with augmented experimental design and meta-analysis (Part 1)

Project description:Non-polar and polar sequential extracts were collected on three groups of C. elegans: natural isolates, central metabolism mutants, and UDP-glucuronosyltrasnferase mutants. Untargeted LC-MS (HILIC/RP - positive and negative mode) and NMR (CDCl3/H2O) studies were conducted using a Vanquish UHPLC coupled to an Orbitrap Elite MS and NEO 800 MHz Bruker NMR spectrometer, respectively. An augmented design, rank transformation of the raw data, strict feature filtering and QA/QC followed by a meta-analysis was performed on all datasets to demonstrate the benefits of this analysis approach and identify stable, significant features to prioritize for downstream compound identification efforts.

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Expression data from CD4 single positive thymocyte subsets from C57BL/6 mice of 6-8 wks of age

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