Metabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting state
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ABSTRACT: Caloric restriction and acute fasting are known to reduce seizures but through unclear mechanisms. In this study, we demonstrate that mTORC1 signaling is reduced after acute fasting of mice. In neurons, mTORC1 is most sensitive to withdrawal of leucine, arginine, and glutamine, which is dependent on DEPDC5. We performed metabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting state. The Depdc5 neuronal specific knockout mice are resistant to sensing significant fluctuations in brain amino acid levels after fasting. These results establish that acute fasting reduces seizure susceptibility in a DEPDC5-dependent manner.
ORGANISM(S): Mouse Mus Musculus
TISSUE(S): Brain
SUBMITTER: Navdeep Chandel
PROVIDER: ST002237 | MetabolomicsWorkbench | Wed Jul 27 00:00:00 BST 2022
REPOSITORIES: MetabolomicsWorkbench
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