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Alterations in SHH signal transduction introduce a state of hypometabolism in sporadic Parkinson's disease

ABSTRACT: Induced pluripotent stem cells (iPSC) derived from sporadic Parkinson's disease patients and healthy control subjects were used for disease modeling. iPSC were differentiated towards midbrain dopaminergic neurons. For metabolic analysis, midbrain neuronal precursor cells were cultivated in growth medium supplemented with either 1.25 mM [U-13C]-glutamine or 21.25 mM [U-13C]-glucose. Metabolites were extracted and analyzed using GC-MS. The MetaboliteDetector software was used to analyze chromatograms, calculate mass isotopomer distributions (MIDs) and perform relative comparison of metabolite levels.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Neurons

DISEASE(S): Parkinsons Disease

SUBMITTER: Sebastian Schmidt

PROVIDER: ST002389 | MetabolomicsWorkbench | Sat Dec 03 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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