Dataset Information
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 1577413149879.gz | Other | |||
| 1577413172604.gz | Other | |||
| 1577413190972.gz | Other | |||
| 1577413211270.gz | Other | |||
| 1577413308673.gz | Other |
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Publications
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.
Nature methods 20200518 6
Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which reveal mutation residues in the predicted DNA-binding site can dramatically decrease the Cas9-independent off-target effects. Furthermore, we obtained a CBE variant-YE1-BE3-FNLS-that retains high on-target editing efficiency while causing extremely low off ...[more]
