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Aspergillus oryzae RIB40

ABSTRACT: Time-course mRNA-Seq analysis of Aspergillus oryzae RIB40 and the faaA disruptant grown in the presence of 1% Triton X-100

PROVIDER: PRJDB8293 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR178650_1.fastq.gz	Fastqsanger.gz
	DRR178650_2.fastq.gz	Fastqsanger.gz
	DRR178651_1.fastq.gz	Fastqsanger.gz
	DRR178651_2.fastq.gz	Fastqsanger.gz
	DRR178652_1.fastq.gz	Fastqsanger.gz

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Aspergillus oryzae RIB40

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Project description:Fungal endo-β-mannanases (β-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of β-mannanases has been identified in fungi or other eukaryotic cells to date. To identify a transcriptional regulator of β-mannanases in Aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced β-mannanase activity by using konjac glucomannan as the substrate, and ManR, a Zn(II)2Cys6 type DNA binding protein was identified. Moreover, a manR-overexpressing strain showed significantly increased β-mannanase activity. DNA microarray analysis of the manR-disruptant strain and the manR-overexpressing strain further indicated that when konjac glucomannan is used as the carbon source, ManR positively regulates the gene expression of not only β-mannanase, but also the enzymes involved in the degradation of galactomannans and glucomannans such as α-galactosidase, β-mannosidase, acetylmannan esterase, and β-glucosidase. Therefore, we conclude that ManR is a positive regulator of the β-mannan utilization system in A. oryzae.

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