Teleogryllus occipitalis
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| BLKR01.dat.gz | Other | |||
| BLKR01.fasta.gz | Fasta.gz | |||
| BLKR01.master.dat | Other | |||
| DRR201339_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 9 |
Similar Datasets
Project description:In Hawaii, a rapidly-evolving mutation in the field cricket Teleogryllus oceanicus silences males by interfering with the development of sound-producing structures on their forewings. The mutation is called flatwing (fw), and it persists because of natural selection imposed by an acoustically-orienting parasitoid. We examined gene expression differences between wild-type and mutant crickets, focusing on juvenile wing buds. We profiled mRNA expression levels using RNA-seq, and characterized the wing bud proteome using quantitative mass spectrometry. Accessing protein expression profiles under the same experimental conditions enabled us to test correspondence between the two ‘omic levels.
2016-04-06 | PXD002417 | Pride
Project description:In Hawaii, a rapidly-evolving mutation in the field cricket Teleogryllus oceanicus silences males by interfering with the development of sound-producing structures on their forewings. The mutation is called flatwing (fw), and it persists because of natural selection imposed by an acoustically-orienting parasitoid. We examined gene expression differences between wild-type and mutant crickets, focusing on juvenile wing buds. We profiled mRNA expression levels using RNA-seq, and characterized the wing bud proteome using quantitative mass spectrometry.
2016-03-31 | PXD002451 | Pride
Project description:Among the parasites of insects, endoparasitoids impose a costly challenge to host defenses because they use their host’s body for the development and maturation of their eggs or larvae, and ultimately kill the host. Tachinid flies are highly specialized acoustically-orienting parasitoids that release first instar mobile larvae which burrow into the host’s body to feed. We investigated the possibility that Teleogryllus oceanicus field crickets employ post-infestation strategies to maximize survival when infested with the larvae of the parasitoid fly Ormia ochracea. Using crickets from the Hawaiian island of Kauai, where the parasitoid is present, and crickets from the Cook Islands (Mangaia), where the parasitoid is absent, we evaluated fitness consequences of infestation by comparing feeding behavior, reproductive capacity, and survival of males experimentally infested with O. ochracea larvae. We also evaluated genetic mechanisms underlying host responses by comparing gene expression in crickets infested with fly larvae for different lengths of time with that of uninfested control crickets. We observe some differences in fitness (spermatophore production) and survival (total survival time post-infestation) between populations. However, for both traits significant population effects 1) were not associated with the slope of the response to different numbers of larvae and 2) only emerged from models containing body condition at one but not both time points evaluated. Gene expression patterns also revealed population differences in response to infestation. We did not find evidence for consistent differences in genes associated with immunity or stress response. Taken together, these results suggest that coevolution with the fly does not strongly select for either post-infestation resistance or tolerance of parasitoid larvae in male crickets.
2020-06-02 | GSE151539 | GEO
Project description:The 1KITE project: evolution of insects
| PRJNA267968 | ENA
Project description:Field crickets (family Gryllidae) frequently are used in studies of behavioral genetics, sexual selection, and sexual conflict, but there have been no studies of transcriptomic differences among different tissue types. We evaluated transcriptome variation among testis, accessory gland, and the remaining whole-body preparations from males of the field cricket, Teleogryllus oceanicus. Non-normalized cDNA libraries from each tissue were sequenced on the Roche 454 platform, and a master assembly was constructed using testis, accessory gland, and whole-body preparations. A total of 940,200 reads were assembled into 41,962 contigs, to which 36,856 singletons (reads not assembled into a contig) were added to provide a total of 78,818 sequences used in annotation analysis. A total of 59,072 sequences (75%) were unique to one of the three tissues. Testis tissue had the greatest proportion of tissue-specific sequences (62.6%), followed by general body (56.43%) and accessory gland tissue (44.16%). We tested the hypothesis that tissues expressing gene products expected to evolve rapidly as a result of sexual selection--testis and accessory gland--would yield a smaller proportion of BLASTx matches to homologous genes in the model organism Drosophila melanogaster compared with whole-body tissue. Uniquely expressed sequences in both testis and accessory gland showed a significantly lower rate of matching to annotated D. melanogaster genes compared with those from general body tissue. These results correspond with empirical evidence that genes expressed in testis and accessory gland tissue are rapidly evolving targets of selection.
| S-EPMC3564983 | biostudies-literature