Bioreactor metagenome
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REPOSITORIES: ENA
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Project description:Prokaryotic community analysis of Cr(VI)-removing culture
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Project description:This study researched the combined effects of hexavalent chromium (Cr(VI)) concentration and antibiotics on the ARGs of Bacillus cereus SH-1. As the Cr(VI) concentration increased, it triggered Reactive Oxygen Species (ROS) oxidative stress in SH-1, increased antioxidant enzyme activity, enhanced plasmid conjugative transfer and reduced the removal efficiency of Cr(VI) by SH-1. Meanwhile, antibiotic resistance varied with TET (Tetracycline) and AMC (Amoxicillin) MICs (minimum inhibitory concentration) rising and AZM (Azithromycin) and CL (Chloramphenicol) MICs decreasing with Cr(VI) induction. Overexpression of eight genes of the HAE-1 family efflux pumps was detected through metagenomics and proteomics. Co-contamination of Cr(VI) and antibiotics led to the emergence and spread of antibiotics resistant bacteria (ARBs).
2024-07-01 | PXD053553 | iProX
Project description:Chromium (Cr) is an important environmental contaminant and also a genotoxic metal ion at high concentration, but its molecular mechanisms and signalling pathways of action in plants is still poorly unraveled. To help characterize the mechanism of molecular and signalling of rice, we present the large-scale, transcriptomic analysis of rice root responses to Cr(VI). We examined the possible involvement of reactive of reactive oxygen species (ROS) and calcium in Cr(VI) signaling transduction pathways, as well as the effect of Cr (VI) on CDPK and MAPK activity. Specially, we used the microarray assay to assess different stage Cr (VI) induced alteration in rice gene expression. This gene discovery effort will help expand our understanding of cellular responses to Cr (VI) treatment, and will identify candidate genes for enhancement of Cr resistance in crop.
2014-12-21 | GSE41733 | GEO
Project description:bioreactor metagenome from a membrane aerated biofilm reactor
| PRJNA598204 | ENA
Project description:Chromium (Cr) is a non-essential metal for normal plants and is toxic to plants at high concentration. In spite of many previous studies having been conducted on the effects of Cr stress, the precise molecular mechanisms and signaling pathways of action of Cr remain poorly understood. In this study, the transcriptome at the early of Cr (VI) stress were assayed in rice roots. To gain more insight into these cellular responses, we analyzed whole-genome transcriptome of rice expose to Cr (VI) for 1 and 3 h. Analysis revealed 1,261 and 267 up and down-regulated genes by Cr (VI). Cr (VI) stress triggered changes in transcript levels of genes related to secondary metabolism process, biosynthetic process, specially jasmonic acid biosynthetic process, response to abiotic stress, specially response to toxin, transcription regulator activity, specially transcription factors activity. The most predominant transcription factor families were WRKY, AP2/ERF, NAC, C2H2, MYB. In addition, many protein kinase, including eight MAPKKK, two CDPK, and one MAPK, showed significant increase in transcriptional level under Cr (VI) stress. Molecular mechanisms for the excess Cr(VI) in rice roots Comparison of mock control and rice seedlings treated with 200 M-NM-<M Cr(VI); Biological replicates: 3 control replicates, 3 Cr(VI)-treated replicates.
2013-10-31 | E-GEOD-33376 | biostudies-arrayexpress