Project description:Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of major S. aureus lineages.
Project description:Here we report a chemically defined strategy to derive the entire human ectoderm from pluripotent stem cells. Using reporter lines for various lineages of the ectoderm, the expression profiles generated were: PAX6+ neuroectoderm (NE), SOX10+ neural crest (NC), SIX1+ cranial placodes (CP). The non-neural ectoderm (NNE) samples did not have a reporter associated, but was quality controlled for the expression of TFAP2A positive, SIX1 and SOX10 negative to proceed with the sequencing. We observe a clear demarcation between the NE versus the non-neural ectoderm lineages (NC, CP and NNE) accompanied by specific expression patterns for the different lineages. The cells with potential to become neurons can be further differentiated to become functional.
Project description:Study hypothesis: The null hypothesis is that intravenous peri- and post-operative fluid restriction does not affect the rate of complications in the first 30 days following major gastrointestinal surgery.
Primary outcome(s): Grade II complications and above up to 30 days post-surgery.
Project description:Gastric bypass surgery (GBP) emerging as a powerful tool for treatment of obesity has been applied for remission of diabetes. However, the GBP global molecular effects on diabetes remission independent of weight loss remain largely unknown. We profiled plasma metabolites and proteins of 10 normoglycemic obese (NO) and 9 diabetic obese (DO) patients at 1-week, 3-months and 1-year stages after Roux-en-Y gastric bypass (RYGB) as well pre-RYGB stage, by which 146 proteins and 128 metabolites were detected from both NO and DO groups at all four stages. By analyzing a set of bi-molecular associations among the corresponding network of the subjects with our newly developed computational method, we defined the representing physiological states (called the edge-states, in contrast to the traditional node-states) and the related molecular networks of NO and DO patients, respectively. The PCA results and hubnetworks of NO subjects were significantly different from those of DO patients; particularly, the hub-network rearrangement of both groups differentially went through after RYGB. In conclusion, by developing network-based systems signatures rather than relying on individual molecules, we for the first time reveal that RYGB generates a unique recovering-path for diabetes remission independent of weight loss.