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Defining the microbial response to colitis through integrated host and microbiome profiling: Replication data set

ABSTRACT: Defining the microbial response to colitis through integrated host and microbiome profiling: Replication data set

PROVIDER: PRJEB11890 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1146190_1.fastq.gz	Fastqsanger.gz
	ERR1146190_2.fastq.gz	Fastqsanger.gz
	ERR1146191_1.fastq.gz	Fastqsanger.gz
	ERR1146191_2.fastq.gz	Fastqsanger.gz
	ERR1146192_1.fastq.gz	Fastqsanger.gz

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Defining the microbial response to colitis through integrated host and microbiome profiling: Replication data set

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Project description:The gut microbiome is significantly altered in inflammatory bowel diseases, but the basis of these changes is not well understood. We have combined metagenomic and metatranscriptomic profiling of the gut microbiome to assess changes to both bacterial community structure and transcriptional activity in a mouse model of colitis. Gene families involved in microbial resistance to oxidative stress, including Dps/ferritin, Fe-dependent peroxidase and glutathione S-transferase, were transcriptionally up-regulated in colitis, implicating a role for increased oxygen tension in gut microbiota modulation. Transcriptional profiling of the host gut tissue and host RNA in the gut lumen revealed a marked increase in the transcription of genes with an activated macrophage and granulocyte signature, suggesting the involvement of these cell types in influencing microbial gene expression. Down-regulation of host glycosylation genes further supports a role for inflammation-driven changes to the gut niche that may impact the microbiome. We propose that members of the bacterial community react to inflammation-associated increased oxygen tension by inducing genes involved in oxidative stress resistance. Furthermore, correlated transcriptional responses between host glycosylation and bacterial glycan utilisation support a role for altered usage of host-derived carbohydrates in colitis. Complementary RNA-seq and DNA-seq data sets of the microbiome from this study have also been deposited at ArrayExpress under accession number E-MTAB-3562 ( http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3562/ ).

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