Project description:The survival of probiotics could be strongly enhanced by delivery vehicles, but the mechanism was unknown. In this study, whole genome microarray technology was used to detect the gene expression profiles of probiotic Lactobacillus casei Zhang in the absence and presence of fermented milk in simulated gastrointestinal tract. The results showed the gene expression profiles were significantly different under these two different conditions. The change of the gene expression profile may be helpful to comprehend the role of delivery vehicles in enhancing the survival of probiotics. Twelve samples of bacterial cells in the absence and presence of fermented milk were collected at 3 h in simulated gastric juice, 4 h and 8 h in intestinal juice, 2 biological replicates were obtained in each time point. The average of the normalized expression values of the 2 biological replicates for each probe was regarded as the expression value of a predicted gene. To identify the expression pattern of each gene across different treatments, n-fold change ratios were calculated for every gene in pure L. casei Zhang versus L. casei Zhang in fermented milk.

2011-12-31 | E-GEOD-23685 | biostudies-arrayexpress

Expression data from Vehicle, MP and MP+CSE_Bu treated SD rats

Project description:Understanding the mechanism of action of CSE-Bu on gene level in osteogenic effect. We used microarray to detail the effect of MP and MP+CSE_Bu on gene expression in SD rats and identified various classes of genes that are either upregulated or down-regulated.

2020-12-31 | GSE143289 | GEO

Gene expression profile of Lactobacillus casei Zhang in fermented milk during transit in simulated gastrointestinal tract

2011-12-31 | GSE23685 | GEO

Muscle and Bone marrow SP and MP cells

Project description:This study identified gene expression of Side Population (SP) and Main Population (MP) cells, isolated from adult murine skeletal muscle and Bone Marrow. Five different preparations of muscle SP, muscle MP, Bone marrw SP and Bone marrow MP cells were used as replicates.

2004-11-16 | GSE1571 | GEO

Methylophaga aminisulfidivorans MP strain:MP(T)

Project description:Methylophaga aminisulfidivorans MP genome sequencing project

| PRJNA66315 | ENA

Insight into Potential Probiotic Markers Predicted in <i>Lactobacillus pentosus</i> MP-10 Genome Sequence.

Project description:Lactobacillus pentosus MP-10 is a potential probiotic lactic acid bacterium originally isolated from naturally fermented Aloreña green table olives. The entire genome sequence was annotated to in silico analyze the molecular mechanisms involved in the adaptation of L. pentosus MP-10 to the human gastrointestinal tract (GIT), such as carbohydrate metabolism (related with prebiotic utilization) and the proteins involved in bacteria-host interactions. We predicted an arsenal of genes coding for carbohydrate-modifying enzymes to modify oligo- and polysaccharides, such as glycoside hydrolases, glycoside transferases, and isomerases, and other enzymes involved in complex carbohydrate metabolism especially starch, raffinose, and levan. These enzymes represent key indicators of the bacteria's adaptation to the GIT environment, since they involve the metabolism and assimilation of complex carbohydrates not digested by human enzymes. We also detected key probiotic ligands (surface proteins, excreted or secreted proteins) involved in the adhesion to host cells such as adhesion to mucus, epithelial cells or extracellular matrix, and plasma components; also, moonlighting proteins or multifunctional proteins were found that could be involved in adhesion to epithelial cells and/or extracellular matrix proteins and also affect host immunomodulation. In silico analysis of the genome sequence of L. pentosus MP-10 is an important initial step to screen for genes encoding for proteins that may provide probiotic features, and thus provides one new routes for screening and studying this potentially probiotic bacterium.

| S-EPMC5439011 | biostudies-literature

OuMV MP-interacting proteins in Arabidopsis

Project description:Co-immunoprecipitation assays coupled with mass spectrometry identified a series of host factors that could interact with the OuMV MP and link the MP with various pathways including membrane trafficking, lipid binding, protein phosphorylation and dephosphorylation, RNA binding, cell wall biosynthesis, blue light response, calcium signaling, transport across the plasma membrane, protein folding and homeostasis, proteolysis, and metabolism.

2022-09-04 | PXD035960 | Pride

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