Project description:Analysis made in 2018 with 25 samples of 15 species of Solanum taken from RFA herbarium (Biology institute - Federal University of Rio de Janeiro - UFRJ)
2021-06-28 | MSV000087711 | GNPS
Project description:Ultra-small bacterial community from Jacarepagua Lagoon, Rio de Janeiro, Brazil
Project description:LC-MS/MS (ESI negative mode) of ethanol extracts of zoanthids P. caribaeorum and P. variabilis collected at beaches from Ceara, Rio de Janeiro and Santa Catarina States, in Brazil
2016-01-21 | MSV000079467 | GNPS
Project description:SARS-CoV-2 genome sequencing Brazil (Amazonas, Rio Grande do Norte, Paraiba, Bahia and Rio de Janeiro)
Project description:Native plants were sampled from nine Atlantic rainforest locations in Brazil for botanical identification, herbarium mounts and chemical analyses. Plants were marked and the coordinate reference determined by Global Positioning System (GPS). Botanical names are as presented in the list of species of Brazilian flora (Lista de Espécies da Flora do Brasil, Jardim Botânico do Rio de Janeiro, 2015). Voucher specimens were deposited at the Herbarium of Instituto Agronômico de Campinas (IAC) (http://herbario.iac.sp.gov.br/), under the given accession numbers (Table 1). Marked plants were resampled in two subsequent years and those failing to be located or in poor condition were excluded from analyses. Environmental data were obtained from meteorological stations at the locations. Monthly values were used to calculate the average seasonal data.
Project description:The established cell lines RT4, 5637 and T24 from human bladder TCC were obtained from the Cell Bank of the Federal University of Rio de Janeiro, Brazil. The 5637 cells harbor two TP53 mutations, at codon 72 (Arg . Pro) and codon 280 (Arg . Thr).T24 cells contain a TP53 allele encoding an in-frame deletion of tyrosine 126. Both cell lines were established from high-grade bladder tumors. No specific mutations were detected in RT4 cells, which had been established from a low-grade papillary bladder tumor. The RT4 and T24 cell lines were maintained in DulbeccoM-^Rs modified EagleM-^Rs medium (Sigma-Aldrich, Inc, St Louis, MO, USA), and the 5637 cells were kept in Roswell Park Memorial Institute medium (Sigma-Aldrich). Both media were supplemented with 10% fetal bovine serum (Cultilab Ltd, Campinas, Brazil), 100 U/mL penicillin G (Sigma-Aldrich), 100 U/mL streptomycin (Sigma-Aldrich) and 1% kanamycin sulfate (Amresco, Branded Products Group, Solon, OH, USA); cells were cultured at 37M-:C in an atmosphere of 5% CO2. A pooled reference design was chosen. More specifically, each array was hybridized with the same reference sample labeled with Cy5, while the experimental samples (control or treated) were labeled with Cy3.
