Identification of the horse dominant curly hair coat gene
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB19479 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR2027931_1.fastq.gz | Fastqsanger.gz | |||
| ERR2027931_2.fastq.gz | Fastqsanger.gz | |||
| ERR2027932_1.fastq.gz | Fastqsanger.gz | |||
| ERR2027932_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 4 of 4 |
Similar Datasets
Project description:A missense mutation in the coil1A domain of the keratin 25 is associated with the dominant curly hair coat trait (Crd) in horse.
| PRJEB19478 | ENA
Project description:Curly horses present a variety of curl phenotypes that are associated with various degrees of curliness of coat, mane, tail and ear hairs. Their origin is still a matter of debate and several genetic hypotheses have been formulated to explain the diversity in phenotype, including the combination of autosomal dominant and recessive alleles. Our purpose was to map the autosomal dominant curly hair locus and identify the causal variant using genome-wide association study (GWAS) and whole-genome sequencing approaches.A GWAS was performed using a Bayesian sparse linear mixed model, based on 51 curly and 19 straight-haired French and North American horses from 13 paternal families genotyped on the Illumina EquineSNP50 BeadChip. A single strong signal was observed on equine chromosome 11, in a region that encompasses the type I keratin gene cluster. This region was refined by haplotype analysis to a segment including 36 genes, among which are 10 keratin genes (KRT-10, -12, -20, -23, -24, -25, -26, -27, -28, -222). To comprehensively identify candidate causal variants within all these genes, whole-genome sequences were obtained for one heterozygous curly stallion and its straight-haired son. Among the four non-synonymous candidate variants identified and validated in the curly region, only variant g.21891160G>A in the KRT25 gene (KRT25:p.R89H) was in perfect agreement with haplotype status in the whole pedigree. Genetic association was then confirmed by genotyping a larger population consisting of 353 horses. However, five discordant curly horses were observed, which carried neither the variant nor the main haplotype associated with curliness. Sequencing of KRT25 for two discordant horses did not identify any other deleterious variant, which suggests locus rather than allelic heterogeneity for the curly phenotype.We identified the KRT25:p.R89H variant as responsible for the dominant curly trait, but a second dominant locus may also be involved in the shape of hairs within North American Curly horses.
| S-EPMC5686958 | biostudies-literature
Project description:Hair shape is defined in the follicle: large hair follicles produce ‘terminal’ hairs, small follicles produce fine ‘vellus’ hairs, curved follicles produce curly hair in all ethnicities, according to the available literature. In order to understand better how shape is determined in the follicle and complement the publically available knowledge on hair shape genetics, our research group undertook a global high-throughput approach to compare the levels of gene expression among straight and very curly hair. The microarray was the methodology chosen. A list of the transcripts differently expressed that pass the statistical two independent groups test including correction for multiple testing (Bonferroni-Hochberg with 0.05 cut-off) was obtained. From 85 genes that were significantly different 68 genes were more expressed in very curly hair follicles and 17 genes were more expressed in straight hair follicles.
2024-02-19 | GSE193983 | GEO
Project description:Comparison of embryo caudal region at E10.5 (28-29 somites) from curly tail (Grhl3 hypomorph) and wild-type (Grhl3 +/+) (97% congenic) embryos.
2020-08-01 | E-MEXP-3673 | biostudies-arrayexpress
Project description:Transcriptional profiling during Arabidopsis seed coat development at 3 key developmental timepoints by using 2 mutant lines and their wild types. The data provides a globe view of seed coat development in arabidopsis can be used for identification of new gene candidates for seed coat development.
2011-03-10 | GSE27857 | GEO
Project description:Petroselinum crispum strain:curly parsley Transcriptome or Gene expression
| PRJNA226641 | ENA
Project description:BACKGROUND:Exposure to horses can cause severe allergic reactions in sensitized individuals. The breed, American Bashkir Curly Horse is categorized as hypoallergenic, primarily due to reports of allergic patients experiencing fewer symptoms while handling this special breed. The possible reasons for this phenomenon could be lower allergen production and/or reduced allergen release into the air because of increased sebum content in their skin and hair compared to other breeds. Therefore, the aim of the current study was to compare different horse breeds in relation to allergen content in hair and airborne dust samples. METHODS:In total, 224 hair samples from 32 different horse breeds were investigated. Personal nasal filters were used to collect airborne dust during the grooming of 20 Curly Horses and 20 Quarter Horses. Quantitative analysis of all samples was performed using two newly developed immunoassays for the detection of horse dander (HD) antigens and the major allergen Equ c 1 and the commercial assay for Equ c 4. Results were analyzed using multiple linear regression models for hair samples and the Mann Whitney U test for airborne samples. RESULTS:Horse antigen and allergen levels differed up to four orders of magnitude between individual animals. Despite enormous variability, levels of HD antigen, Equ c 1 and Equ c 4 in hair were significantly related to the breed and gender combined with the castration status of male animals. Curly Horses had significantly higher concentrations of all three tested parameters compared to the majority of the investigated breeds (medians: 11800 ?g/g for HD antigen, 2400 ?g/g for Equ c 1, and 258 kU/g for Equ c 4). Tinker Horses, Icelandic Horses and Shetland Ponies were associated with approximately 7-fold reduced levels of HD antigen and Equ c 1, and up to 25-fold reduced levels of Equ c 4 compared to Curly Horses. Compared to mares, stallions displayed increased concentrations of HD antigens, Equ c 1 and Equ c 4 by a factor 2.2, 3.5 and 6.7, respectively. No difference was observed between mares and geldings. No differences in airborne allergen concentrations collected with personal nasal filters during grooming were found between Curly and Quarter Horses. CONCLUSION:Breed and castration status had a significant influence on the antigen and allergen levels of horse hair. However, these differences were smaller than the wide variability observed among individual horses. Compared to other breeds, Curly Horses were not associated with lower allergen levels in hair and in air samples collected during grooming. Our approach provides no molecular explanation why Curly Horses are considered to be hypoallergenic.
| S-EPMC6291085 | biostudies-literature
Project description:Transcriptional profiling during Arabidopsis seed coat development at 3 key developmental timepoints by using 2 mutant lines and their wild types. The data provides a globe view of seed coat development in arabidopsis can be used for identification of new gene candidates for seed coat development. 3 seed coat development stages, 4 lines (2 wild type + 2 mutants) of arabidopsis were sampled. 4 biological replicates.
2011-03-10 | E-GEOD-27857 | biostudies-arrayexpress
Project description:Soybean cultivars, OX951 & OX736, comparison of seed coat at 3 growth stages, early, mid & late Keywords: time course
2007-01-01 | GSE4276 | GEO
Project description:Transcriptional analysis of stigmas was performed to identify molecules functioning in compatible pollination. Stigmas of A. thaliana Col-0 were collected (i) at 0 min or 15 min after pollination and (ii) before or at 15 min after pollen coat adhesion, and used for microarray analysis. Genes up-regulated after pollination and after pollen coat adhesion were identified. .
2014-02-26 | GSE54637 | GEO