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The impact of conflicting selection on the trajectory of bacterial molecular evolution

ABSTRACT: Experimental evolution using Pseudomonas fluorescens SBW25, its plasmid pQBR103 and bacteriophage SBW25phi2 to investigate impact of tripartite interactions on bacterial evolution

PROVIDER: PRJEB19606 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1854980_1.fastq.gz	Fastqsanger.gz
	ERR1854980_2.fastq.gz	Fastqsanger.gz
	ERR1854990_1.fastq.gz	Fastqsanger.gz
	ERR1854990_2.fastq.gz	Fastqsanger.gz
	ERR1854971_1.fastq.gz	Fastqsanger.gz

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Plasmid manipulation of bacterial behaviour through translational regulatory crosstalk

Project description:pQBR103 is a naturally occurring plasmid known to alter the behaviour of its host, Pseudomonas fluorescens. This plasmid encodes a predicted translational regulator, rsmQ which is a homologue of known global regulators within Pseudomonas. Within this study we compared Pseudomonas fluorescens SBW25 plasmid free cells with cells carrying either WT pQBR103 or pQBR103 lacking rsmQ. Using comparative quantitative proteomics and RNAseq we showed that the loss of rsmQ leads to widespread proteomic changes in the absence of changes in mRNA abundance, suggesting RsmQ is a translational regulator of its host chromosome.

2023-01-13 | PXD033640 | Pride

RNA-Seq of Pseudomonas fluorescens SBW25 with and without the plasmid pQBR103 and with the rsmQ deleted from pQBR103 to determine the effect of the plasmid on global gene transcription.

Project description:Plasmids can affect expression of genes on the host chromosome. This experiment was carried out to determine the effect of the presence of the plasmid pQBR103 (with and without the rsmQ gene on it) on global gene expression in Pseudomonas fluorescens SBW25.

2022-12-15 | E-MTAB-11868 | biostudies-arrayexpress

Expression analysis of the gacS mutant of Pseudomonas fluorescens SBW25

Project description:Pseudomonas species are ubiquitous in plant-associated environments and produce an array of volatiles, enzymes and antimicrobials. The biosynthesis of many metabolites is regulated by the GacS/GacA two-component regulatory system. Transcriptome analysis of Pseudomonas fluorescens SBW25 revealed that 702 genes were differentially regulated (fold change>4, P<0.0001) in a gacS::Tn5 mutant, with 300 and 402 genes up- and down-regulated, respectively. Genes that were significantly down-regulated are involved in viscosin biosynthesis (viscABC), protease production (aprA), motility, biofilm formation, and secretory systems. Genes that were significantly up-regulated are involved in siderophore biosynthesis and oxidative stress. In contrast to previous studies with gac-mutants of other Pseudomonas species/strains, the gacS mutant of SBW25 inhibited growth of oomycete, fungal and bacterial pathogens significantly more than parental strain SBW25. A potential candidate for this enhanced antimicrobial activity was a large nonribosomal peptide synthetase (NRPS) gene cluster predicted to encode for an 8-amino-acid ornicorrugatin-like peptide. Site-directed mutagenesis of an NRPS gene in this cluster, however, did not lead to a reduction in the antimicrobial activity of the gacS mutant. Collectively these results indicate that a mutation in the GacS/GacA regulatory system causes major transcriptional changes in P. fluorescens SBW25 and significantly enhances its antimicrobial activities by yet unknown mechanisms. This expression study used total RNA recovered from four separate wild-type cultures of Pseudomonas fluorescens SBW25 and four separate cultures of the gacS mutant. Expression design was based on the updated genome sequence of Pseudomonas fluorescens SBW25, NC_012660.1 and associated plasmid pQBR0476 with nineteen 60-mer probe per gene. Each probe is replicated 3 times. The design includes random GC and other control probes.

2013-06-17 | E-GEOD-43443 | biostudies-arrayexpress

RNA-Seq of Pseudomonas fluorescens SBW25 isolated from axenic rhizospheres of the barley varieties Chevallier and Tipple one and five days post-inoculation.

Project description:Pseudomonas fluorescens SBW25 cultures were inoculated into the rhizospheres of barley seedlings of the Chevallier and Tipple varieties growing in axenic cultures. Bacterial cells were collected from the rhizosphere one and five days after inculation and RNA extracted from them. Culture used for inoculation (but not exposed to the rhizospheres) were used as control. The aim of the experiment was to determine the changes in gene expression of P. fluorescens SBW25 upon exposure to barley rhizosphere and also to determine if the rhizospehres of the two varieties of Barley had different effects on gene expression of P. fluorescens SBW25.

2023-05-15 | E-MTAB-12918 | biostudies-arrayexpress

Analysis of the Hfq regulon in Pseudomonas fluorescens SBW25.

Project description:Hfq is a transcriptional and translational pleiotropic regulator in several bacteria. RNA-Seq, Ribo-Seq and Proteomic analyses were carried out in the wild-type and a hfq deletion strain of Pseudomonas fluorescens SBW25 with the intention to separate the influence of Hfq on the transcript stability and translation. This submission relates to the RNA-Seq data only. RNA was extracted from two replicate cultures each of SBW25-WT and SBW25-Δhfq strains and, after removal of ribosomal RNA, subjected to RNA-Seq in an Illumina NextSeq500 machine. The resulting sequence data was analysed by mapping to the reference sequence of Pseudomonas fluorescens SBW25 as available in the Genbank accession NC_012660.

2019-02-07 | E-MTAB-5396 | biostudies-arrayexpress

RNA-seq data showing the effects of rpoD mutation t1682c in Pseudomonas fluorescens

Project description:We report RNA-seq data for four Pseudomonas fluorescens SBW25 types: SBW25 (wild type) and dervied types 6A4, 6B4-Cap- and 6B4-Cap+. The aim of the study is to see the effect of a mutation in rpoD (carried by 6B4) on the transcriptome.

2019-03-01 | GSE116490 | GEO

Ribosome profiling of Pseudomonas fluorescens SBW25 and its hfq deletion mutant.

Project description:Hfq is a transcriptional and translational pleiotropic regulator in several bacteria. RNA-Seq, Ribo-Seq and Proteomic analyses were carried out in the wild-type and a hfq deletion strain of Pseudomonas fluorescens SBW25 with the intention to separate the influence of Hfq on the transcript stability and translation. This submission relates to the Ribosome profiling data only. Ribosomes were purified from MNase treated cell lysates by density gradient ultracentrifugation from two replicate cultures each of SBW25-WT and SBW25-hfq strains. RNA was extracted from the purified ribosomes and subjected to RNA-Seq in an Illumina HiSeq2000 machine. The resulting sequence data was analysed by mapping to the reference sequence of Pseudomonas fluorescens SBW25 as available in the Genbank accession NC_012660.

2017-08-24 | E-MTAB-5408 | biostudies-arrayexpress

RNAseq data from wild type and evolved Pseudomonas fluorescens SBW25 strains

Project description:We report mRNA expression data from Pseudomonas fluorescens SBW25 wild type and two evolved strains (Beaumont et al., 2009). The evolution of one of these strains saw the emergence of colony switching; 1B4 switches rapidly between two different colony phenotypes. These two phenotypes were found to be genetically identical. Thus, in order to gain insight into epigenetic mechanisms of switching, we were interested in identifiying gene expression differences between ancestors and the 1B4 colony phenotypes.

2015-02-05 | GSE48900 | GEO

Iron deficiency response of interactions between Brachypodium and Pseudomonas fluorescens SBW25 under controlled growth conditions.

Project description:The goal of this study was to evaluate the molecular mechanisms by which Brachypodium distachyon grown with and without Pseudomonas fluorescens (P. fluorescens) strain SBW25 respond to Fe deprivation. Fe deprivation induced Brachypodium secretion of phytosiderophores and reduced biomass production while inoculation with P. fluorescens resulted in alterations of extracellular metabolite abundances. Results provide insight into the role of iron in interactions between a host plant and root associated bacteria.

2020-09-22 | GSE158273 | GEO

Amelioration of plasmid carriage

Project description:Experimental evolution using Pseudomonas fluorescens SBW25 and its plasmid pQBR103 to investigate costs of plasmid carriage and compensatory mutations to ameliorate these costs

| PRJEB8710 | ENA

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